Saeid Abediankenari1, Maryam Ghasemi2, Young-June Kim3. 1. Dept. of Microbiology and Immunology, Mazandaran University of Medical Sciences, Sari, Iran. 2. Dept. of Pathology, Faculty of Medicine, Mazandaran zzm321990University of Medical Sciences, Sari, Iran. 3. Dept. of Microbiology and Immunology, zzm321990Walter Oncology Center, Indianapolis, Indiana, USA.
Abstract
BACKGROUND: During antigen capture and processing, mature dendritic cells (DC) express large amounts of peptide-MHC complexes and accessory molecules on their surface. DC are antigen-presenting cells that have an important role in tolerance and autoimmunity. The transforming growth factor-beta1 (TGF-Beta1) cytokine has a regulatory role on the immune and non-immune cells. The aim of this study is to evaluate the effect of TGF-Beta1 on the induction of human leukocyte antigen-G (HLA-G) expression on the DC which is derived from monocyte. METHODS: In this study, we evaluated the effect of TGF-Beta1 in induction HLA-G expression on the monocyte-derived DC by flowcytometry and then CD4+ T cell proliferative responses in the presence of DC-treated TGF-Beta1 was studied. RESULTS: The results of this study showed that DC bearing HLA-G down-regulated activation of CD4+ T cells and production of IL-6 and IL-17 in comparison with control (P<0.05). CONCLUSION: It is concluded that TGF-Beta1 has an important regulatory role in CD4+ T cell proliferation by increasing HLA-G on DC and these cells can probably prevent unexpected immune responses in vivo.
BACKGROUND: During antigen capture and processing, mature dendritic cells (DC) express large amounts of peptide-MHC complexes and accessory molecules on their surface. DC are antigen-presenting cells that have an important role in tolerance and autoimmunity. The transforming growth factor-beta1 (TGF-Beta1) cytokine has a regulatory role on the immune and non-immune cells. The aim of this study is to evaluate the effect of TGF-Beta1 on the induction of human leukocyte antigen-G (HLA-G) expression on the DC which is derived from monocyte. METHODS: In this study, we evaluated the effect of TGF-Beta1 in induction HLA-G expression on the monocyte-derived DC by flowcytometry and then CD4+ T cell proliferative responses in the presence of DC-treated TGF-Beta1 was studied. RESULTS: The results of this study showed that DC bearing HLA-G down-regulated activation of CD4+ T cells and production of IL-6 and IL-17 in comparison with control (P<0.05). CONCLUSION: It is concluded that TGF-Beta1 has an important regulatory role in CD4+ T cell proliferation by increasing HLA-G on DC and these cells can probably prevent unexpected immune responses in vivo.
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