| Literature DB >> 2170022 |
R A Katz1, G Merkel, J Kulkosky, J Leis, A M Skalka.
Abstract
The integration of viral DNA into the host cell chromosome is an essential feature of the retroviral life cycle. The integration reaction requires cis-acting sequences at the ends of linear viral DNA and a trans-acting product of the pol gene, the integration protein (IN). Previously, we demonstrated that avian sarcoma-leukosis virus (ASLV) IN is able to carry out the first step in the integration process in vitro: nicking of the ends of linear viral DNA. In this paper, using two independent assays, we demonstrate that IN, alone, is sufficient to carry out the second step: cleavage and joining to the target DNA. These results demonstrate that the retroviral IN protein is an integrase.Entities:
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Year: 1990 PMID： 2170022 DOI： 10.1016/0092-8674(90)90290-u
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582