Literature DB >> 10196350

Monoclonal antibodies against the minimal DNA-binding domain in the carboxyl-terminal region of human immunodeficiency virus type 1 integrase.

T Ishikawa1, N Okui, N Kobayashi, R Sakuma, T Kitamura, Y Kitamura.   

Abstract

Integrase of human immunodeficiency virus type 1 (HIVIN) consists of 288 amino acids, and its minimum DNA-binding domain (MDBD) (amino acids [aa] 220 to 270) is required for the integration reaction. We produced and characterized four murine monoclonal antibodies (MAbs) to the MDBD of HIVIN (strain LAI). Immunoblot and enzyme-linked immunosorbent assays with truncated HIVINs showed that those MAbs recognized sequential epitopes within the MDBD (aa 228 to 236, 237 to 252, 253 to 261, and 262 to 270). Their binding to HIVIN inhibited terminal cleavage and strand transfer activities but not disintegration activity in vitro. This collection of MAbs is useful for studying the structure and function of the MDBD by complementing mutational analyses and other biochemical studies.

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Year:  1999        PMID： 10196350      PMCID： PMC104339     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  38 in total

1.  A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.

Authors:  J F Kearney; A Radbruch; B Liesegang; K Rajewsky
Journal:  J Immunol       Date:  1979-10       Impact factor: 5.422

2.  Complete nucleotide sequence of the AIDS virus, HTLV-III.

Authors:  L Ratner; W Haseltine; R Patarca; K J Livak; B Starcich; S F Josephs; E R Doran; J A Rafalski; E A Whitehorn; K Baumeister
Journal:  Nature       Date:  1985 Jan 24-30       Impact factor: 49.962

3.  Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.

Authors:  A Adachi; H E Gendelman; S Koenig; T Folks; R Willey; A Rabson; M A Martin
Journal:  J Virol       Date:  1986-08       Impact factor: 5.103

4.  Structure-based mutational analysis of the C-terminal DNA-binding domain of human immunodeficiency virus type 1 integrase: critical residues for protein oligomerization and DNA binding.

Authors:  R A Lutzke; R H Plasterk
Journal:  J Virol       Date:  1998-06       Impact factor: 5.103

5.  Human recombinant antibody fragments neutralizing human immunodeficiency virus type 1 reverse transcriptase provide an experimental basis for the structural classification of the DNA polymerase family.

Authors:  N Gargano; S Biocca; A Bradbury; A Cattaneo
Journal:  J Virol       Date:  1996-11       Impact factor: 5.103

6.  Site-directed mutagenesis of HIV-1 integrase demonstrates differential effects on integrase functions in vitro.

Authors:  A D Leavitt; L Shiue; H E Varmus
Journal:  J Biol Chem       Date:  1993-01-25       Impact factor: 5.157

7.  Inhibition of human immunodeficiency virus type 1 integrase by the Fab fragment of a specific monoclonal antibody suggests that different multimerization states are required for different enzymatic functions.

Authors:  E V Barsov; W E Huber; J Marcotrigiano; P K Clark; A D Clark; E Arnold; S H Hughes
Journal:  J Virol       Date:  1996-07       Impact factor: 5.103

8.  Identification of the catalytic and DNA-binding region of the human immunodeficiency virus type I integrase protein.

Authors:  C Vink; A M Oude Groeneger; R H Plasterk
Journal:  Nucleic Acids Res       Date:  1993-03-25       Impact factor: 16.971

9.  Human immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate viral DNA efficiently during infection.

Authors:  A D Leavitt; G Robles; N Alesandro; H E Varmus
Journal:  J Virol       Date:  1996-02       Impact factor: 5.103

10.  Production of uninfectious human immunodeficiency virus type 1 containing viral protein R fused to a single-chain antibody against viral integrase.

Authors:  N Okui; N Kobayashi; Y Kitamura
Journal:  J Virol       Date:  1998-08       Impact factor: 5.103
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  9 in total

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Authors:  Iris Oz; Orna Avidan; Amnon Hizi
Journal:  Biochem J       Date:  2002-02-01       Impact factor: 3.857

2.  Correlation of recombinant integrase activity and functional preintegration complex formation during acute infection by replication-defective integrase mutant human immunodeficiency virus.

Authors:  Xiang Li; Yasuhiro Koh; Alan Engelman
Journal:  J Virol       Date:  2012-01-25       Impact factor: 5.103

3.  Structural dynamics of native and V260E mutant C-terminal domain of HIV-1 integrase.

Authors:  Balasubramanian Sangeetha; Rajagopalan Muthukumaran; Ramaswamy Amutha
Journal:  J Comput Aided Mol Des       Date:  2015-01-14       Impact factor: 3.686

4.  Cooperation between reverse transcriptase and integrase during reverse transcription and formation of the preintegrative complex of Ty1.

Authors:  Marcelle Wilhelm; F-X Wilhelm
Journal:  Eukaryot Cell       Date:  2006-10

5.  Role of integrase in reverse transcription of the Saccharomyces cerevisiae retrotransposon Ty1.

Authors:  M Wilhelm; F-X Wilhelm
Journal:  Eukaryot Cell       Date:  2005-06

Review 6.  Multimodal Functionalities of HIV-1 Integrase.

Authors:  Alan N Engelman; Mamuka Kvaratskhelia
Journal:  Viruses       Date:  2022-04-28       Impact factor: 5.818

7.  Replication of chimeric human immunodeficiency virus type 1 (HIV-1) containing HIV-2 integrase (IN): naturally selected mutations in IN augment DNA synthesis.

Authors:  Marcus Padow; Lilin Lai; Champion Deivanayagam; Lawrence J DeLucas; Robert B Weiss; Diane M Dunn; Xiaoyun Wu; John C Kappes
Journal:  J Virol       Date:  2003-10       Impact factor: 5.103

8.  Recombinant rabbit single-chain antibodies bind to the catalytic and C-terminal domains of HIV-1 integrase protein and strongly inhibit HIV-1 replication.

Authors:  Frederico Aires da Silva; Min Li; Sylvie Rato; Sara Maia; Rui Malhó; Kylie Warren; David Harrich; Robert Craigie; Carlos Barbas; Joao Goncalves
Journal:  Biotechnol Appl Biochem       Date:  2012-10-10       Impact factor: 2.431

9.  Interaction between human immunodeficiency virus type 1 reverse transcriptase and integrase proteins.

Authors:  Eric A Hehl; Pheroze Joshi; Ganjam V Kalpana; Vinayaka R Prasad
Journal:  J Virol       Date:  2004-05       Impact factor: 5.103
  9 in total

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