| Literature DB >> 21690406 |
Mary O'Connell Motherway1, Aldert Zomer, Sinead C Leahy, Justus Reunanen, Francesca Bottacini, Marcus J Claesson, Frances O'Brien, Kiera Flynn, Patrick G Casey, Jose Antonio Moreno Munoz, Breda Kearney, Aileen M Houston, Caitlin O'Mahony, Des G Higgins, Fergus Shanahan, Airi Palva, Willem M de Vos, Gerald F Fitzgerald, Marco Ventura, Paul W O'Toole, Douwe van Sinderen.
Abstract
Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tad(2003)." Mutational analysis demonstrated that the tad(2003) gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria.Entities:
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Year: 2011 PMID： 21690406 PMCID： PMC3131351 DOI： 10.1073/pnas.1105380108
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205