OBJECTIVES: While screening the activity of potential inhibitors of matrix metalloproteinases (MMPs), due to the limited water solubility of some of the compounds, they had to be solubilized in ethanol. When ethanol solvent controls were run, they were found to partially inhibit MMPs. Thus, the purpose of this study was to compare the MMP-inhibitory activity of a series of alcohols. METHODS: The possible inhibitory activity of a series of alcohols was measured against soluble rhMMP-9 and insoluble matrix-bound endogenous MMPs of dentin in completely demineralized dentin. Increasing concentrations (0.17, 0.86, 1.71 and 4.28 mol/L) of a homologous series of alcohols (i.e. methanol, ethanol, propanols, butanols, pentanols, hexanols, the ethanol ester of methacrylic acid, heptanols and octanol) were compared to ethanediol, and propanediol by regression analysis to calculate the molar concentration required to inhibit MMPs by 50% (i.e. the IC(50)). RESULTS: Using two different MMP models, alcohols were shown to inhibit rhMMP-9 and the endogenous proteases of dentin matrix in a dose-dependent manner. The degree of MMP inhibition by alcohols increased with chain length up to 4 methylene groups. Based on the molar concentration required to inhibit rhMMP-9 fifty percent, 2-hydroxyethylmethacrylate (HEMA), 3-hexanol, 3-heptanol and 1-octanol gave the strongest inhibition. SIGNIFICANCE: The results indicate that alcohols with 4 methylene groups inhibit MMPs more effectively than methanol or ethanol. MMP inhibition was inversely related to the Hoy's solubility parameter for hydrogen bonding forces of the alcohols (i.e. to their hydrophilicity).
OBJECTIVES: While screening the activity of potential inhibitors of matrix metalloproteinases (MMPs), due to the limited water solubility of some of the compounds, they had to be solubilized in ethanol. When ethanol solvent controls were run, they were found to partially inhibit MMPs. Thus, the purpose of this study was to compare the MMP-inhibitory activity of a series of alcohols. METHODS: The possible inhibitory activity of a series of alcohols was measured against soluble rhMMP-9 and insoluble matrix-bound endogenous MMPs of dentin in completely demineralized dentin. Increasing concentrations (0.17, 0.86, 1.71 and 4.28 mol/L) of a homologous series of alcohols (i.e. methanol, ethanol, propanols, butanols, pentanols, hexanols, the ethanol ester of methacrylic acid, heptanols and octanol) were compared to ethanediol, and propanediol by regression analysis to calculate the molar concentration required to inhibit MMPs by 50% (i.e. the IC(50)). RESULTS: Using two different MMP models, alcohols were shown to inhibit rhMMP-9 and the endogenous proteases of dentin matrix in a dose-dependent manner. The degree of MMP inhibition by alcohols increased with chain length up to 4 methylene groups. Based on the molar concentration required to inhibit rhMMP-9 fifty percent, 2-hydroxyethylmethacrylate (HEMA), 3-hexanol, 3-heptanol and 1-octanol gave the strongest inhibition. SIGNIFICANCE: The results indicate that alcohols with 4 methylene groups inhibit MMPs more effectively than methanol or ethanol. MMP inhibition was inversely related to the Hoy's solubility parameter for hydrogen bonding forces of the alcohols (i.e. to their hydrophilicity).
Authors: J De Munck; B Van Meerbeek; Y Yoshida; S Inoue; M Vargas; K Suzuki; P Lambrechts; G Vanherle Journal: J Dent Res Date: 2003-02 Impact factor: 6.116
Authors: Arzu Tezvergil-Mutluay; Kelli A Agee; Tomohiro Hoshika; Marcela Carrilho; Lorenzo Breschi; Leo Tjäderhane; Yoshihiro Nishitani; Ricardo M Carvalho; Stephen Looney; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2010-08-04 Impact factor: 5.304
Authors: Leo Tjäderhane; Fabio D Nascimento; Lorenzo Breschi; Annalisa Mazzoni; Ivarne L S Tersariol; Saulo Geraldeli; Arzu Tezvergil-Mutluay; Marcela Carrilho; Ricardo M Carvalho; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2013-08-14 Impact factor: 5.304
Authors: Camila Sabatini; Débora L S Scheffel; Régis H Scheffel; Kelli A Agee; Katelyn Rouch; Masahiro Takahashi; Lorenzo Breschi; Annalisa Mazzoni; Leo Tjäderhane; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2014-05-17 Impact factor: 5.304
Authors: Débora L S Scheffel; Josimeri Hebling; Régis H Scheffel; Kelli A Agee; Milena Cadenaro; Gianluca Turco; Lorenzo Breschi; Annalisa Mazzoni; Carlos A de Souza Costa; David H Pashley Journal: Dent Mater Date: 2013-12-13 Impact factor: 5.304
Authors: Walter G Renné; Amanda Lindner; Anthony S Mennito; Kelli A Agee; David H Pashley; Daniel Willett; David Sentelle; Michael Defee; Michael Schmidt; Camila Sabatini Journal: Clin Oral Investig Date: 2016-03-28 Impact factor: 3.573