Camila Sabatini1, Débora L S Scheffel2, Régis H Scheffel2, Kelli A Agee3, Katelyn Rouch3, Masahiro Takahashi4, Lorenzo Breschi5, Annalisa Mazzoni6, Leo Tjäderhane7, Franklin R Tay8, David H Pashley9. 1. Department of Restorative Dentistry, School of Dental Medicine, University at Buffalo, Buffalo, NY, USA. Electronic address: cs252@buffalo.edu. 2. Department of Orthodontics and Pediatric Dentistry, Universidade Estadual Paulista - UNESP, Araraquara Dental School, Araraquara, São Paulo, Brazil. 3. Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA. 4. Department of Cariology and Operative Dentistry, Tokyo Medical and Dental University, Tokyo, Japan. 5. Department of Biomedicine, University of Trieste, Trieste, Italy; IGM-CNR, Unit of Bologna c/o IOR, Bologna, Italy. 6. Department of SAU&FAL, University of Bologna, Bologna, Italy. 7. Institute of Dentistry, University of Oulu and Oulu University Hospital, Oulu, Finland. 8. Department of Endodontics, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA. 9. Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA; Highly Cited Investigator of King Abdulazis University School of Dentistry, Jeddah, Saudi Arabia.
Abstract
OBJECTIVE: The objective of this study was to determine if Gluma dentin desensitizer (5.0% glutaraldehyde and 35% HEMA in water) can inhibit the endogenous MMPs of dentin matrices in 60 s and to evaluate its effect on dentin matrix stiffness and dry mass weight. METHODS: Dentin beams of 2 mm×1 mm×6 mm were obtained from extracted human third molars coronal dentin. To measure the influence of Gluma treatment time on total MMP activity of dentin, beams were dipped in 37% phosphoric acid (PA) for 15 s and rinsed in water. The acid-etched beams were then dipped in Gluma for 5, 15, 30 or 60 s, rinsed in water and incubated into SensoLyte generic MMP substrate (AnaSpec, Inc.) for 60 min. Controls were dipped in water for 60 s. Additional beams of 1 mm×1 mm×6 mm were completely demineralized in 37% PA for 18 h, rinsed and used to evaluate changes on the dry weight and modulus of elasticity (E) after 60 s of Gluma treatment followed by incubation in simulated body fluid buffer for 0, 1 or 4 weeks. E was measured by 3-pt flexure. RESULTS: Gluma treatment inhibited total MMP activity of acid-etched dentin by 44, 50, 84, 86% after 5, 15, 30 or 60 s of exposure, respectively. All completely demineralized dentin beams lost stiffness after 1 and 4 weeks, with no significant differences between the control and Gluma-treated dentin. Gluma treatment for 60 s yielded significantly less dry mass loss than the control after 4 weeks. SIGNIFICANCE: The use of Gluma may contribute to the preservation of adhesive interfaces by its cross-linking and inhibitory properties of endogenous dentin MMPs.
OBJECTIVE: The objective of this study was to determine if Gluma dentin desensitizer (5.0% glutaraldehydeand 35% HEMA in water) can inhibit the endogenous MMPs of dentin matrices in 60 s and to evaluate its effect on dentin matrix stiffness and dry mass weight. METHODS: Dentin beams of 2 mm×1 mm×6 mm were obtained from extracted human third molars coronal dentin. To measure the influence of Gluma treatment time on total MMP activity of dentin, beams were dipped in 37% phosphoric acid (PA) for 15 s andrinsed in water. The acid-etched beams were then dipped in Gluma for 5, 15, 30 or 60 s, rinsed in waterand incubated into SensoLyte generic MMP substrate (AnaSpec, Inc.) for 60 min. Controls were dipped in water for 60 s. Additional beams of 1 mm×1 mm×6 mm were completely demineralized in 37% PA for 18 h, rinsed and used to evaluate changes on the dry weight and modulus of elasticity (E) after 60 s of Gluma treatment followed by incubation in simulated body fluid buffer for 0, 1 or 4 weeks. E was measured by 3-pt flexure. RESULTS:Gluma treatment inhibited total MMP activity of acid-etched dentin by 44, 50, 84, 86% after 5, 15, 30 or 60 s of exposure, respectively. All completely demineralized dentin beams lost stiffness after 1 and 4 weeks, with no significant differences between the control andGluma-treated dentin. Gluma treatment for 60 s yielded significantly less dry mass loss than the control after 4 weeks. SIGNIFICANCE: The use of Gluma may contribute to the preservation of adhesive interfaces by its cross-linking and inhibitory properties of endogenous dentin MMPs.
Authors: P M C Scaffa; C M P Vidal; N Barros; T F Gesteira; A K Carmona; L Breschi; D H Pashley; L Tjäderhane; I L S Tersariol; F D Nascimento; M R Carrilho Journal: J Dent Res Date: 2012-01-19 Impact factor: 6.116
Authors: T Ingman; T Tervahartiala; Y Ding; H Tschesche; A Haerian; D F Kinane; Y T Konttinen; T Sorsa Journal: J Clin Periodontol Date: 1996-12 Impact factor: 8.728
Authors: Leo Tjäderhane; Fabio D Nascimento; Lorenzo Breschi; Annalisa Mazzoni; Ivarne L S Tersariol; Saulo Geraldeli; Arzu Tezvergil-Mutluay; Marcela R Carrilho; Ricardo M Carvalho; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2012-08-16 Impact factor: 5.304