Walter G Renné1, Amanda Lindner2, Anthony S Mennito1, Kelli A Agee3, David H Pashley3,4, Daniel Willett5, David Sentelle2, Michael Defee2, Michael Schmidt6, Camila Sabatini7. 1. Department of Oral Rehabilitation and Restorative Dentistry, College of Dental Medicine, Medical University of South Carolina, 173 Ashley Ave, Charleston, SC, 29425, USA. 2. Department of Oral Health Sciences, College of Dental Medicine, Medical University of South Carolina, 173 Ashley Ave, Charleston, SC, 29425, USA. 3. Department of Oral Biology, College of Dental Medicine, Georgia Regents University, 1120 15th Street, Augusta, GA, 30912, USA. 4. Highly Cited Investigator of King Abdulazis University School of Dentistry, Jeddah, Saudi Arabia. 5. Department of Chemistry, Clemson University, 116 Biosystems Research Complex, Clemson, SC, 29634, USA. 6. Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Ave, MSC504, Charleston, SC, 29425-0504, USA. 7. Department of Restorative Dentistry, School of Dental Medicine, State University of New York at Buffalo, 3435 Main Street, Buffalo, NY, 14214, USA. cs252@buffalo.edu.
Abstract
OBJECTIVES: This study investigated the antibacterial properties and micro-hardness of polyacrylic acid (PAA)-coated copper iodide (CuI) nanoparticles incorporated into glass ionomer-based materials, and the effect of PAA-CuI on collagen degradation. MATERIALS AND METHODS: PAA-CuI nanoparticles were incorporated into glass ionomer (GI), Ionofil Molar AC, and resin-modified glass ionomer (RMGI), Vitrebond, at 0.263 wt%. The antibacterial properties against Streptococcus mutans (n = 6/group) and surface micro-hardness (n = 5/group) were evaluated. Twenty dentin beams were completely demineralized in 10 wt% phosphoric acid and equally divided in two groups (n = 10/group) for incubation in simulated body fluid (SBF) or SBF containing 1 mg/ml PAA-CuI. The amount of dry mass loss and hydroxyproline (HYP) released were quantified. Kruskal-Wallis, Student's t test, two-way ANOVA, and Mann-Whitney were used to analyze the antibacterial, micro-hardness, dry mass, and HYP release data, respectively (p < 0.05). RESULTS: Addition of PAA-CuI nanoparticles into the glass ionomer matrix yielded significant reduction (99.999 %) in the concentration of bacteria relative to the control groups. While micro-hardness values of PAA-CuI-doped GI were no different from its control, PAA-CuI-doped RMGI demonstrated significantly higher values than its control. A significant decrease in dry mass weight was shown only for the control beams (10.53 %, p = 0.04). Significantly less HYP was released from beams incubated in PAA-CuI relative to the control beams (p < 0.001). CONCLUSIONS: PAA-CuI nanoparticles are an effective additive to glass ionomer-based materials as they greatly enhance their antibacterial properties and reduce collagen degradation without an adverse effect on their mechanical properties. CLINICAL RELEVANCE: The use of copper-doped glass ionomer-based materials under composite restorations may contribute to an increased longevity of adhesive restorations, because of their enhanced antibacterial properties and reduced collagen degradation.
OBJECTIVES: This study investigated the antibacterial properties and micro-hardness of polyacrylic acid (PAA)-coated copper iodide (CuI) nanoparticles incorporated into glass ionomer-based materials, and the effect of PAA-CuI on collagen degradation. MATERIALS AND METHODS:PAA-CuI nanoparticles were incorporated into glass ionomer (GI), Ionofil Molar AC, and resin-modified glass ionomer (RMGI), Vitrebond, at 0.263 wt%. The antibacterial properties against Streptococcus mutans (n = 6/group) and surface micro-hardness (n = 5/group) were evaluated. Twenty dentin beams were completely demineralized in 10 wt% phosphoric acid and equally divided in two groups (n = 10/group) for incubation in simulated body fluid (SBF) or SBF containing 1 mg/ml PAA-CuI. The amount of dry mass loss and hydroxyproline (HYP) released were quantified. Kruskal-Wallis, Student's t test, two-way ANOVA, and Mann-Whitney were used to analyze the antibacterial, micro-hardness, dry mass, and HYP release data, respectively (p < 0.05). RESULTS: Addition of PAA-CuI nanoparticles into the glass ionomer matrix yielded significant reduction (99.999 %) in the concentration of bacteria relative to the control groups. While micro-hardness values of PAA-CuI-doped GI were no different from its control, PAA-CuI-doped RMGI demonstrated significantly higher values than its control. A significant decrease in dry mass weight was shown only for the control beams (10.53 %, p = 0.04). Significantly less HYP was released from beams incubated in PAA-CuI relative to the control beams (p < 0.001). CONCLUSIONS:PAA-CuI nanoparticles are an effective additive to glass ionomer-based materials as they greatly enhance their antibacterial properties and reduce collagen degradation without an adverse effect on their mechanical properties. CLINICAL RELEVANCE: The use of copper-doped glass ionomer-based materials under composite restorations may contribute to an increased longevity of adhesive restorations, because of their enhanced antibacterial properties and reduced collagen degradation.
Authors: Catherine Fan; Lianrui Chu; H Ralph Rawls; Barry K Norling; Hector L Cardenas; Kyumin Whang Journal: Dent Mater Date: 2010-11-26 Impact factor: 5.304
Authors: Anthony S Mennito; Michael Schmidt; Andrew Lane; Abigail Kelly; Camila Sabatini; Walter Renne; Zachary Evans Journal: Contemp Clin Dent Date: 2022-06-21
Authors: Elena Ferrando-Magraner; Carlos Bellot-Arcís; Vanessa Paredes-Gallardo; José Manuel Almerich-Silla; Verónica García-Sanz; Mercedes Fernández-Alonso; José María Montiel-Company Journal: Medicina (Kaunas) Date: 2020-01-29 Impact factor: 2.430