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Literature DB >> 21670958

DNA barcoding of nymphalid butterflies (Nymphalidae: Lepidoptera) from Western Ghats of India.

S S Gaikwad¹, H V Ghate, S S Ghaskadbi, M S Patole, Y S Shouche.

Abstract

We have checked the utility of DNA barcoding for species identification of nymphalid butterflies from Western Ghats of India by using 650 bp sequence of mitochondrial gene cytochrome c oxidase subunit I. Distinct DNA barcoding gap (i.e. difference between intraspecies and interspecies nucleotide divergence), exists between species studied here. When our sequences were compared with the sequences of the conspecifics submitted from different geographic regions, nine cases of deep intraspecies nucleotide divergences were observed. In spite of this, NJ (Neighbour Joining) clustering analysis successfully discriminated all species. Observed cases of deep intraspecies nucleotide divergences certainly warrant further study.

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Year: 2011 PMID： 21670958 DOI： 10.1007/s11033-011-0988-7

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

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