Gil M Yerushalmi1, Ettie Maman, Yuval Yung, Alon Kedem, Ariel Hourvitz. 1. IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel. gil.yerushalmi@gmail.com
Abstract
AIMS: Ovarian follicular development and ovulation in mammals is a complex and highly regulated process. Most advances in the understanding of the ovulatory process have come from animal models. However, translational research in humans is of crucial importance for improving fertility treatment and control. METHODS: IVM/IVF procedures allow us to obtain follicular fluid and granulosa cells (GC) from follicles in different developmental stages with and without hCG priming. RESULTS: Using the cells and fluids obtained in IVM/IVF procedures allowed us to characterize human ovulatory gene expression during antral folliculogenesis and ovulation, examine gene expression in luteinized and non-luteinized GC in vivo and in vitro and to use cumulus GC genes as biomarkers for oocyte and embryo maturity and competence. CONCLUSION: Biological material obtained during IVM/IVF procedures is an important tool to study the human ovulatory cascade and can serve to improve IVM techniques and fertility treatment and control.
AIMS: Ovarian follicular development and ovulation in mammals is a complex and highly regulated process. Most advances in the understanding of the ovulatory process have come from animal models. However, translational research in humans is of crucial importance for improving fertility treatment and control. METHODS: IVM/IVF procedures allow us to obtain follicular fluid and granulosa cells (GC) from follicles in different developmental stages with and without hCG priming. RESULTS: Using the cells and fluids obtained in IVM/IVF procedures allowed us to characterize human ovulatory gene expression during antral folliculogenesis and ovulation, examine gene expression in luteinized and non-luteinized GC in vivo and in vitro and to use cumulus GC genes as biomarkers for oocyte and embryo maturity and competence. CONCLUSION: Biological material obtained during IVM/IVF procedures is an important tool to study the human ovulatory cascade and can serve to improve IVM techniques and fertility treatment and control.
Authors: R L Robker; D L Russell; S Yoshioka; S C Sharma; J P Lydon; B W O'Malley; L L Espey; J S Richards Journal: Steroids Date: 2000 Oct-Nov Impact factor: 2.668
Authors: Feng-Tao Shi; Anthony P Cheung; Christian Klausen; He-Feng Huang; Peter C K Leung Journal: J Clin Endocrinol Metab Date: 2010-07-21 Impact factor: 5.958
Authors: R Fadini; M B Dal Canto; M Mignini Renzini; F Brambillasca; R Comi; D Fumagalli; M Lain; M Merola; R Milani; E De Ponti Journal: Reprod Biomed Online Date: 2009-09 Impact factor: 3.828
Authors: L J McKenzie; S A Pangas; S A Carson; E Kovanci; P Cisneros; J E Buster; P Amato; M M Matzuk Journal: Hum Reprod Date: 2004-10-07 Impact factor: 6.918
Authors: A Hourvitz; E Gershon; J D Hennebold; S Elizur; E Maman; C Brendle; E Y Adashi; N Dekel Journal: J Endocrinol Date: 2006-03 Impact factor: 4.286
Authors: Joanna Budna; Piotr Celichowski; Artur Bryja; Marta Dyszkiewicz-Konwińska; Michal Jeseta; Dorota Bukowska; Paweł Antosik; Klaus Peter Brüssow; Małgorzata Bruska; Michał Nowicki; Maciej Zabel; Bartosz Kempisty Journal: Int J Mol Sci Date: 2017-12-11 Impact factor: 5.923
Authors: Joanna Budna; Marta Rybska; Sylwia Ciesiółka; Artur Bryja; Sylwia Borys; Wiesława Kranc; Katarzyna Wojtanowicz-Markiewicz; Michal Jeseta; Ewa Sumelka; Dorota Bukowska; Paweł Antosik; Klaus P Brüssow; Małgorzata Bruska; Michał Nowicki; Maciej Zabel; Bartosz Kempisty Journal: Reprod Biol Endocrinol Date: 2017-06-02 Impact factor: 5.211