Literature DB >> 2166232

Synthesis of the herpes simplex virus type 1 trans-activator Vmw65 in insect cells using a baculovirus vector.

J P Capone1, G Werstuck.   

Abstract

Vmw65, the Herpes Simplex Virus trans-activator of immediate-early genes, was expressed in insect cells using a recombinant baculovirus expression vector and partially purified. Insect cell-derived Vmw65 was shown to be indistinguishable from authentic Vmw65 present in purified HSV-1 virions based on electrophoretic mobility, immunoreactivity with a monoclonal antibody, and ability to interact with cellular factors to form a protein/DNA complex with oligonucleotides containing a TAATGARAT element.

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Year:  1990        PMID： 2166232     DOI： 10.1007/BF00223561

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  20 in total

1.  Mutational analysis of the herpes simplex virus trans-inducing factor Vmw65.

Authors:  G Werstuck; J P Capone
Journal:  Gene       Date:  1989-02-20       Impact factor: 3.688

2.  Screening recombinant baculovirus plaques in situ with antibody probes.

Authors:  J Capone
Journal:  Gene Anal Tech       Date:  1989 May-Jun

3.  Evidence of DNA: protein interactions that mediate HSV-1 immediate early gene activation by VP16.

Authors:  S J Triezenberg; K L LaMarco; S L McKnight
Journal:  Genes Dev       Date:  1988-06       Impact factor: 11.361

4.  A herpesvirus trans-activating protein interacts with transcription factor OTF-1 and other cellular proteins.

Authors:  T Gerster; R G Roeder
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

5.  A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.

Authors:  H Singh; R Sen; D Baltimore; P A Sharp
Journal:  Nature       Date:  1986 Jan 9-15       Impact factor: 49.962

6.  DNA sequence of the herpes simplex virus type 1 gene whose product is responsible for transcriptional activation of immediate early promoters.

Authors:  M A Dalrymple; D J McGeoch; A J Davison; C M Preston
Journal:  Nucleic Acids Res       Date:  1985-11-11       Impact factor: 16.971

7.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

8.  Identification of herpes simplex virus DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcription.

Authors:  M E Campbell; J W Palfreyman; C M Preston
Journal:  J Mol Biol       Date:  1984-11-25       Impact factor: 5.469

9.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

10.  Signals important for high-level expression of foreign genes in Autographa californica nuclear polyhedrosis virus expression vectors.

Authors:  V A Luckow; M D Summers
Journal:  Virology       Date:  1988-11       Impact factor: 3.616
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