Literature DB >> 21642963

Sharper low-power STED nanoscopy by time gating.

Giuseppe Vicidomini1, Gael Moneron, Kyu Y Han, Volker Westphal, Haisen Ta, Matthias Reuss, Johann Engelhardt, Christian Eggeling, Stefan W Hell.   

Abstract

Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.

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Year:  2011        PMID： 21642963     DOI： 10.1038/nmeth.1624

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  15 in total

1.  Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission.

Authors:  T A Klar; S Jakobs; M Dyba; A Egner; S W Hell
Journal:  Proc Natl Acad Sci U S A       Date:  2000-07-18       Impact factor: 11.205

2.  Nanoscale resolution in the focal plane of an optical microscope.

Authors:  Volker Westphal; Stefan W Hell
Journal:  Phys Rev Lett       Date:  2005-04-15       Impact factor: 9.161

3.  Macromolecular-scale resolution in biological fluorescence microscopy.

Authors:  Gerald Donnert; Jan Keller; Rebecca Medda; M Alexandra Andrei; Silvio O Rizzoli; Reinhard Lührmann; Reinhard Jahn; Christian Eggeling; Stefan W Hell
Journal:  Proc Natl Acad Sci U S A       Date:  2006-07-24       Impact factor: 11.205

4.  Microscopy and its focal switch.

Authors:  Stefan W Hell
Journal:  Nat Methods       Date:  2009-01       Impact factor: 28.547

5.  Three-dimensional stimulated emission depletion microscopy of nitrogen-vacancy centers in diamond using continuous-wave light.

Authors:  Kyu Young Han; Katrin I Willig; Eva Rittweger; Fedor Jelezko; Christian Eggeling; Stefan W Hell
Journal:  Nano Lett       Date:  2009-09       Impact factor: 11.189

6.  Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.

Authors:  S W Hell; J Wichmann
Journal:  Opt Lett       Date:  1994-06-01       Impact factor: 3.776

7.  Fast STED microscopy with continuous wave fiber lasers.

Authors:  Gael Moneron; Rebecca Medda; Birka Hein; Arnold Giske; Volker Westphal; Stefan W Hell
Journal:  Opt Express       Date:  2010-01-18       Impact factor: 3.894

8.  Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications.

Authors:  O Griesbeck; G S Baird; R E Campbell; D A Zacharias; R Y Tsien
Journal:  J Biol Chem       Date:  2001-05-31       Impact factor: 5.157

9.  Direct observation of the nanoscale dynamics of membrane lipids in a living cell.

Authors:  Christian Eggeling; Christian Ringemann; Rebecca Medda; Günter Schwarzmann; Konrad Sandhoff; Svetlana Polyakova; Vladimir N Belov; Birka Hein; Claas von Middendorff; Andreas Schönle; Stefan W Hell
Journal:  Nature       Date:  2008-12-21       Impact factor: 49.962

10.  hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.

Authors:  Philippe Lamesch; Ning Li; Stuart Milstein; Changyu Fan; Tong Hao; Gabor Szabo; Zhenjun Hu; Kavitha Venkatesan; Graeme Bethel; Paul Martin; Jane Rogers; Stephanie Lawlor; Stuart McLaren; Amélie Dricot; Heather Borick; Michael E Cusick; Jean Vandenhaute; Ian Dunham; David E Hill; Marc Vidal
Journal:  Genomics       Date:  2007-01-05       Impact factor: 5.736
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  105 in total

1.  Single-wavelength two-photon excitation-stimulated emission depletion (SW2PE-STED) superresolution imaging.

Authors:  Paolo Bianchini; Benjamin Harke; Silvia Galiani; Giuseppe Vicidomini; Alberto Diaspro
Journal:  Proc Natl Acad Sci U S A       Date:  2012-04-09       Impact factor: 11.205

2.  Visualization of the immunological synapse by dual color time-gated stimulated emission depletion (STED) nanoscopy.

Authors:  Emily M Mace; Jordan S Orange
Journal:  J Vis Exp       Date:  2014-03-24       Impact factor: 1.355

Review 3.  Super-resolution microscopy for analyzing neuromuscular junctions and synapses.

Authors:  Yomna Badawi; Hiroshi Nishimune
Journal:  Neurosci Lett       Date:  2019-11-22       Impact factor: 3.046

4.  Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution.

Authors:  Fabian Göttfert; Christian A Wurm; Veronika Mueller; Sebastian Berning; Volker C Cordes; Alf Honigmann; Stefan W Hell
Journal:  Biophys J       Date:  2013-07-02       Impact factor: 4.033

5.  Gated STED microscopy with time-gated single-photon avalanche diode.

Authors:  Iván Coto Hernández; Mauro Buttafava; Gianluca Boso; Alberto Diaspro; Alberto Tosi; Giuseppe Vicidomini
Journal:  Biomed Opt Express       Date:  2015-05-27       Impact factor: 3.732

Review 6.  Super-resolution microscopy approaches for live cell imaging.

Authors:  Antoine G Godin; Brahim Lounis; Laurent Cognet
Journal:  Biophys J       Date:  2014-10-21       Impact factor: 4.033

7.  STED imaging performance estimation by means of Fourier transform analysis.

Authors:  David Merino; Arrate Mallabiabarrena; Jordi Andilla; David Artigas; Timo Zimmermann; Pablo Loza-Alvarez
Journal:  Biomed Opt Express       Date:  2017-04-17       Impact factor: 3.732

8.  STAQ: A route toward low power, multicolor nanoscopy.

Authors:  Tilman Rosales; Dan L Sackett; Jianhua Xu; Zhen-Dan Shi; Biying Xu; Haitao Li; Gurpreet Kaur; Erin Frohart; Nalini Shenoy; Sarah M Cheal; Haitao Wu; Andrés E Dulcey; Yulin Hu; Changhui Li; Kelly Lane; Gary L Griffiths; Jay R Knutson
Journal:  Microsc Res Tech       Date:  2015-03-12       Impact factor: 2.769

9.  Increasing fluorescence lifetime for resolution improvement in stimulated emission depletion nanoscopy.

Authors:  Lu-Wei Wang; Yue Chen; Wei Yan; Xiao-Yu Weng; Zhi-Gang Yang; Tong Ye; Jun-Le Qu
Journal:  J Biophotonics       Date:  2019-01-02       Impact factor: 3.207

10.  Richardson-Lucy deconvolution as a general tool for combining images with complementary strengths.

Authors:  Maria Ingaramo; Andrew G York; Eelco Hoogendoorn; Marten Postma; Hari Shroff; George H Patterson
Journal:  Chemphyschem       Date:  2014-01-16       Impact factor: 3.102
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