Literature DB >> 23823248

Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution.

Fabian Göttfert, Christian A Wurm, Veronika Mueller, Sebastian Berning, Volker C Cordes, Alf Honigmann, Stefan W Hell.   

Abstract

We report on a fiber laser-based stimulated emission-depletion microscope providing down to ∼20 nm resolution in raw data images as well as 15-19 nm diameter probing areas in fluorescence correlation spectroscopy. Stimulated emission depletion pulses of nanosecond duration and 775 nm wavelength are used to silence two fluorophores simultaneously, ensuring offset-free colocalization analysis. The versatility of this superresolution method is exemplified by revealing the octameric arrangement of Xenopus nuclear pore complexes and by quantifying the diffusion of labeled lipid molecules in artificial and living cell membranes.
Copyright © 2013 Biophysical Society. Published by Elsevier Inc. All rights reserved.

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Mesh：

Year:  2013        PMID： 23823248      PMCID： PMC3699760          DOI： 10.1016/j.bpj.2013.05.029

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  16 in total

1.  Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission.

Authors:  T A Klar; S Jakobs; M Dyba; A Egner; S W Hell
Journal:  Proc Natl Acad Sci U S A       Date:  2000-07-18       Impact factor: 11.205

2.  Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution.

Authors:  Anna Löschberger; Sebastian van de Linde; Marie-Christine Dabauvalle; Bernd Rieger; Mike Heilemann; Georg Krohne; Markus Sauer
Journal:  J Cell Sci       Date:  2012-02-01       Impact factor: 5.285

3.  Two-color far-field fluorescence nanoscopy.

Authors:  Gerald Donnert; Jan Keller; Christian A Wurm; Silvio O Rizzoli; Volker Westphal; Andreas Schönle; Reinhard Jahn; Stefan Jakobs; Christian Eggeling; Stefan W Hell
Journal:  Biophys J       Date:  2007-02-16       Impact factor: 4.033

4.  Sample preparation for STED microscopy.

Authors:  Christian A Wurm; Daniel Neumann; Roman Schmidt; Alexander Egner; Stefan Jakobs
Journal:  Methods Mol Biol       Date:  2010

5.  Immunocytochemistry of annulate lamellae: potential cell biological markers for studies of cell differentiation and pathology.

Authors:  V C Cordes; A Gajewski; S Stumpp; G Krohne
Journal:  Differentiation       Date:  1995-04       Impact factor: 3.880

6.  STED nanoscopy with mass-produced laser diodes.

Authors:  Susanne Schrof; Thorsten Staudt; Eva Rittweger; Nina Wittenmayer; Thomas Dresbach; Johann Engelhardt; Stefan W Hell
Journal:  Opt Express       Date:  2011-04-25       Impact factor: 3.894

7.  Time-gating improves the spatial resolution of STED microscopy.

Authors:  Jeffrey R Moffitt; Christian Osseforth; Jens Michaelis
Journal:  Opt Express       Date:  2011-02-28       Impact factor: 3.894

8.  STED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue.

Authors:  Alf Honigmann; Veronika Mueller; Stefan W Hell; Christian Eggeling
Journal:  Faraday Discuss       Date:  2013       Impact factor: 4.008

9.  STED nanoscopy reveals molecular details of cholesterol- and cytoskeleton-modulated lipid interactions in living cells.

Authors:  V Mueller; C Ringemann; A Honigmann; G Schwarzmann; R Medda; M Leutenegger; S Polyakova; V N Belov; S W Hell; C Eggeling
Journal:  Biophys J       Date:  2011-10-05       Impact factor: 4.033

10.  Direct observation of the nanoscale dynamics of membrane lipids in a living cell.

Authors:  Christian Eggeling; Christian Ringemann; Rebecca Medda; Günter Schwarzmann; Konrad Sandhoff; Svetlana Polyakova; Vladimir N Belov; Birka Hein; Claas von Middendorff; Andreas Schönle; Stefan W Hell
Journal:  Nature       Date:  2008-12-21       Impact factor: 49.962
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  79 in total

Review 1.  Super-resolution microscopy for analyzing neuromuscular junctions and synapses.

Authors:  Yomna Badawi; Hiroshi Nishimune
Journal:  Neurosci Lett       Date:  2019-11-22       Impact factor: 3.046

2.  Pex35 is a regulator of peroxisome abundance.

Authors:  Ido Yofe; Kareem Soliman; Silvia G Chuartzman; Bruce Morgan; Uri Weill; Eden Yifrach; Tobias P Dick; Sara J Cooper; Christer S Ejsing; Maya Schuldiner; Einat Zalckvar; Sven Thoms
Journal:  J Cell Sci       Date:  2017-01-03       Impact factor: 5.285

Review 3.  Time-resolved fluorescence in lipid bilayers: selected applications and advantages over steady state.

Authors:  Mariana Amaro; Radek Šachl; Piotr Jurkiewicz; Ana Coutinho; Manuel Prieto; Martin Hof
Journal:  Biophys J       Date:  2014-12-16       Impact factor: 4.033

4.  Gated STED microscopy with time-gated single-photon avalanche diode.

Authors:  Iván Coto Hernández; Mauro Buttafava; Gianluca Boso; Alberto Diaspro; Alberto Tosi; Giuseppe Vicidomini
Journal:  Biomed Opt Express       Date:  2015-05-27       Impact factor: 3.732

5.  Rab3-interacting molecules 2α and 2β promote the abundance of voltage-gated CaV1.3 Ca2+ channels at hair cell active zones.

Authors:  Sangyong Jung; Tomoko Oshima-Takago; Rituparna Chakrabarti; Aaron B Wong; Zhizi Jing; Gulnara Yamanbaeva; Maria Magdalena Picher; Sonja M Wojcik; Fabian Göttfert; Friederike Predoehl; Katrin Michel; Stefan W Hell; Susanne Schoch; Nicola Strenzke; Carolin Wichmann; Tobias Moser
Journal:  Proc Natl Acad Sci U S A       Date:  2015-06-01       Impact factor: 11.205

Review 6.  Nanoscale invaginations of the nuclear envelope: Shedding new light on wormholes with elusive function.

Authors:  Ingmar Schoen; Lina Aires; Jonas Ries; Viola Vogel
Journal:  Nucleus       Date:  2017-07-07       Impact factor: 4.197

7.  Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent.

Authors:  Fabian Göttfert; Tino Pleiner; Jörn Heine; Volker Westphal; Dirk Görlich; Steffen J Sahl; Stefan W Hell
Journal:  Proc Natl Acad Sci U S A       Date:  2017-02-13       Impact factor: 11.205

Review 8.  Applications of STED fluorescence nanoscopy in unravelling nanoscale structure and dynamics of biological systems.

Authors:  C Roobala; I P Ilanila; J K Basu
Journal:  J Biosci       Date:  2018-07       Impact factor: 1.826

9.  Increasing fluorescence lifetime for resolution improvement in stimulated emission depletion nanoscopy.

Authors:  Lu-Wei Wang; Yue Chen; Wei Yan; Xiao-Yu Weng; Zhi-Gang Yang; Tong Ye; Jun-Le Qu
Journal:  J Biophotonics       Date:  2019-01-02       Impact factor: 3.207

10.  Active zone scaffolds differentially accumulate Unc13 isoforms to tune Ca(2+) channel-vesicle coupling.

Authors:  Mathias A Böhme; Christina Beis; Suneel Reddy-Alla; Eric Reynolds; Malou M Mampell; Andreas T Grasskamp; Janine Lützkendorf; Dominique Dufour Bergeron; Jan H Driller; Husam Babikir; Fabian Göttfert; Iain M Robinson; Cahir J O'Kane; Stefan W Hell; Markus C Wahl; Ulrich Stelzl; Bernhard Loll; Alexander M Walter; Stephan J Sigrist
Journal:  Nat Neurosci       Date:  2016-08-15       Impact factor: 24.884
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