Literature DB >> 21642404

Identifying vulnerable pathways in Mycobacterium tuberculosis by using a knockdown approach.

Paul Carroll1, Marie-Claire Faray-Kele, Tanya Parish.   

Abstract

We constructed recombinant strains of Mycobacterium tuberculosis in which expression of specific genes was downregulated to identify vulnerable drug targets. Growth phenotypes in macrophages and culture were used to rank targets: the dprE1, clpP1, and fadD32 operons were the best targets and glnA1, glnE, pknL, regX3, and senX3 were poor targets.

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Year:  2011        PMID： 21642404      PMCID： PMC3147394          DOI： 10.1128/AEM.02880-10

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  The transcriptional responses of Mycobacterium tuberculosis to inhibitors of metabolism: novel insights into drug mechanisms of action.

Authors:  Helena I M Boshoff; Timothy G Myers; Brent R Copp; Michael R McNeil; Michael A Wilson; Clifton E Barry
Journal:  J Biol Chem       Date:  2004-07-09       Impact factor: 5.157

2.  Electroporation of mycobacteria.

Authors:  Renan Goude; Tanya Parish
Journal:  Methods Mol Biol       Date:  2009

3.  Rapid recombination screening to test gene essentiality demonstrates that pyrH is essential in Mycobacterium tuberculosis.

Authors:  Dina Robertson; Paul Carroll; Tanya Parish
Journal:  Tuberculosis (Edinb)       Date:  2007-08-24       Impact factor: 3.131

4.  Potential drug targets in Mycobacterium tuberculosis through metabolic pathway analysis.

Authors:  Sharmila Anishetty; Mrudula Pulimi; Gautam Pennathur
Journal:  Comput Biol Chem       Date:  2005-10-06       Impact factor: 2.877

5.  High extracellular levels of Mycobacterium tuberculosis glutamine synthetase and superoxide dismutase in actively growing cultures are due to high expression and extracellular stability rather than to a protein-specific export mechanism.

Authors:  M V Tullius; G Harth; M A Horwitz
Journal:  Infect Immun       Date:  2001-10       Impact factor: 3.441

6.  Use of a tetracycline-inducible system for conditional expression in Mycobacterium tuberculosis and Mycobacterium smegmatis.

Authors:  Paul Carroll; D G Niranjala Muttucumaru; Tanya Parish
Journal:  Appl Environ Microbiol       Date:  2005-06       Impact factor: 4.792

7.  Glutamine synthetase GlnA1 is essential for growth of Mycobacterium tuberculosis in human THP-1 macrophages and guinea pigs.

Authors:  Michael V Tullius; Günter Harth; Marcus A Horwitz
Journal:  Infect Immun       Date:  2003-07       Impact factor: 3.441

8.  Functional analysis of GlnE, an essential adenylyl transferase in Mycobacterium tuberculosis.

Authors:  Paul Carroll; Carey A Pashley; Tanya Parish
Journal:  J Bacteriol       Date:  2008-05-09       Impact factor: 3.490

9.  Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Authors:  Samiul Hasan; Sabine Daugelat; P S Srinivasa Rao; Mark Schreiber
Journal:  PLoS Comput Biol       Date:  2006-06-09       Impact factor: 4.475

10.  targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.

Authors:  Karthik Raman; Kalidas Yeturu; Nagasuma Chandra
Journal:  BMC Syst Biol       Date:  2008-12-19
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  25 in total

Review 1.  Adenylating enzymes in Mycobacterium tuberculosis as drug targets.

Authors:  Benjamin P Duckworth; Kathryn M Nelson; Courtney C Aldrich
Journal:  Curr Top Med Chem       Date:  2012       Impact factor: 3.295

2.  A genetic strategy to identify targets for the development of drugs that prevent bacterial persistence.

Authors:  Jee-Hyun Kim; Kathryn M O'Brien; Ritu Sharma; Helena I M Boshoff; German Rehren; Sumit Chakraborty; Joshua B Wallach; Mercedes Monteleone; Daniel J Wilson; Courtney C Aldrich; Clifton E Barry; Kyu Y Rhee; Sabine Ehrt; Dirk Schnappinger
Journal:  Proc Natl Acad Sci U S A       Date:  2013-11-04       Impact factor: 11.205

3.  Insight into Structure-Function Relationships and Inhibition of the Fatty Acyl-AMP Ligase (FadD32) Orthologs from Mycobacteria.

Authors:  Valérie Guillet; Ségolène Galandrin; Laurent Maveyraud; Simon Ladevèze; Vincent Mariaule; Cécile Bon; Nathalie Eynard; Mamadou Daffé; Hedia Marrakchi; Lionel Mourey
Journal:  J Biol Chem       Date:  2016-02-21       Impact factor: 5.157

4.  The Protease Locus of Francisella tularensis LVS Is Required for Stress Tolerance and Infection in the Mammalian Host.

Authors:  Lihong He; Manoj Kumar Mohan Nair; Yuling Chen; Xue Liu; Mengyun Zhang; Karsten R O Hazlett; Haiteng Deng; Jing-Ren Zhang
Journal:  Infect Immun       Date:  2016-04-22       Impact factor: 3.441

Review 5.  Bacterial proteases, untapped antimicrobial drug targets.

Authors:  Elizabeth Culp; Gerard D Wright
Journal:  J Antibiot (Tokyo)       Date:  2016-11-30       Impact factor: 2.649

6.  Diarylcoumarins inhibit mycolic acid biosynthesis and kill Mycobacterium tuberculosis by targeting FadD32.

Authors:  Sarah A Stanley; Tomohiko Kawate; Noriaki Iwase; Motohisa Shimizu; Anne E Clatworthy; Edward Kazyanskaya; James C Sacchettini; Thomas R Ioerger; Noman A Siddiqi; Shoko Minami; John A Aquadro; Sarah Schmidt Grant; Eric J Rubin; Deborah T Hung
Journal:  Proc Natl Acad Sci U S A       Date:  2013-06-24       Impact factor: 11.205

7.  Ser/Thr Phosphorylation Regulates the Fatty Acyl-AMP Ligase Activity of FadD32, an Essential Enzyme in Mycolic Acid Biosynthesis.

Authors:  Nguyen-Hung Le; Virginie Molle; Nathalie Eynard; Mathieu Miras; Alexandre Stella; Fabienne Bardou; Ségolène Galandrin; Valérie Guillet; Gwenaëlle André-Leroux; Marco Bellinzoni; Pedro Alzari; Lionel Mourey; Odile Burlet-Schiltz; Mamadou Daffé; Hedia Marrakchi
Journal:  J Biol Chem       Date:  2016-09-02       Impact factor: 5.157

8.  Regulated Expression Systems for Mycobacteria and Their Applications.

Authors:  Dirk Schnappinger; Sabine Ehrt
Journal:  Microbiol Spectr       Date:  2014

9.  Pathway-selective sensitization of Mycobacterium tuberculosis for target-based whole-cell screening.

Authors:  Garth L Abrahams; Anuradha Kumar; Suzana Savvi; Alvin W Hung; Shijun Wen; Chris Abell; Clifton E Barry; David R Sherman; Helena I M Boshoff; Valerie Mizrahi
Journal:  Chem Biol       Date:  2012-07-27

10.  Structure of the Essential Mtb FadD32 Enzyme: A Promising Drug Target for Treating Tuberculosis.

Authors:  Misty L Kuhn; Evan Alexander; George Minasov; Holland J Page; Zdzislaw Warwrzak; Ludmilla Shuvalova; Kristin J Flores; Daniel J Wilson; Ce Shi; Courtney C Aldrich; Wayne F Anderson
Journal:  ACS Infect Dis       Date:  2016-07-01       Impact factor: 5.084
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