Literature DB >> 21632019

False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011.

D M Whiley1, A Limnios, N J Moon, N Gehrig, N Goire, T Hogan, A Lam, K Jacob, S B Lambert, M D Nissen, T P Sloots.   

Abstract

The gonococcal porA pseudogene is a popular target for in-house Neisseria gonorrhoeae PCR methods. With this study we present two novel findings: the first case of an N. gonorrhoeae porA pseudogene PCR false-negative result caused by sequence variation, and in the same organism, the first description of a clinical N. gonorrhoeae strain harbouring an N. meningitidis porA sequence.

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Year:  2011        PMID： 21632019

Source DB:  PubMed          Journal:  Euro Surveill        ISSN： 1025-496X


  14 in total

1.  Supplementary testing is not required in the cobas 4800 CT/NG test for Neisseria gonorrhoeae weak-positive urogenital samples.

Authors:  Collette Bromhead; Nadika Liyanarachchy; Julia Mayes; Arlo Upton; Michelle Balm
Journal:  J Clin Microbiol       Date:  2014-11-12       Impact factor: 5.948

2.  Evaluation of the New BD Max GC Real-Time PCR Assay, Analytically and Clinically as a Supplementary Test for the BD ProbeTec GC Qx Amplified DNA Assay, for Molecular Detection of Neisseria gonorrhoeae.

Authors:  Daniel Golparian; Stina Boräng; Martin Sundqvist; Magnus Unemo
Journal:  J Clin Microbiol       Date:  2015-10-14       Impact factor: 5.948

3.  Molecular Assay for Detection of Genetic Markers Associated with Decreased Susceptibility to Cephalosporins in Neisseria gonorrhoeae.

Authors:  S W Peterson; I Martin; W Demczuk; A Bharat; L Hoang; J Wylie; V Allen; B Lefebvre; G Tyrrell; G Horsman; D Haldane; R Garceau; T Wong; M R Mulvey
Journal:  J Clin Microbiol       Date:  2015-04-15       Impact factor: 5.948

4.  Evaluation of six commercial nucleic acid amplification tests for detection of Neisseria gonorrhoeae and other Neisseria species.

Authors:  Sepehr N Tabrizi; Magnus Unemo; Athena E Limnios; Tiffany R Hogan; Stig-Ove Hjelmevoll; Susanne M Garland; John Tapsall
Journal:  J Clin Microbiol       Date:  2011-08-03       Impact factor: 5.948

5.  Analytical evaluation of GeneXpert CT/NG, the first genetic point-of-care assay for simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis.

Authors:  Sepehr N Tabrizi; Magnus Unemo; Daniel Golparian; Jimmy Twin; Athena E Limnios; Monica Lahra; Rebecca Guy
Journal:  J Clin Microbiol       Date:  2013-04-03       Impact factor: 5.948

6.  Purification of DNA eluates from the ProbeTec GC Q(x) assay on BD Viper™ XTR allows for further analysis and confirmation of gonorrhea.

Authors:  H S Tunsjø; E Smedsrud; P C Holm; K Rokvam; C Schie; V Rognlien; I Augustin; A Fostervold
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2013-08-01       Impact factor: 3.267

7.  Molecular epidemiology of selected sexually transmitted infections.

Authors:  Hamid Jalal; Andrew Delaney; Neil Bentley; Christopher Sonnex; Christopher A Carne
Journal:  Int J Mol Epidemiol Genet       Date:  2013-09-12

8.  Use of the mtrR Gene for Rapid Molecular Diagnosis of Neisseria gonorrhoeae and Identification of the Reduction of Susceptibility to Antibiotics in Endocervical Swabs.

Authors:  Marcos R Escobedo-Guerra; Mitzuko Katoku-Herrera; Marcela Lopez-Hurtado; Rodrigo Gutierrez-Trujillo; Fernando M Guerra-Infante
Journal:  Mol Diagn Ther       Date:  2018-06       Impact factor: 4.074

9.  Neisseria meningitidis Lacking the Major Porins PorA and PorB Is Viable and Modulates Apoptosis and the Oxidative Burst of Neutrophils.

Authors:  Ian R Peak; Adrienne Chen; Freda E-C Jen; Courtney Jennings; Benjamin L Schulz; Nigel J Saunders; Arshad Khan; H Steven Seifert; Michael P Jennings
Journal:  J Proteome Res       Date:  2016-07-21       Impact factor: 4.466

Review 10.  Towards multitarget testing in molecular microbiology.

Authors:  Deborah Steensels; Anne Vankeerberghen; Hans De Beenhouwer
Journal:  Int J Microbiol       Date:  2013-11-25
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