Literature DB >> 2161937

Enhancer functions in U3 of Akv virus: a role for cooperativity of a tandem repeat unit and its flanking DNA sequences.

S Lovmand1, N O Kjeldgaard, P Jørgensen, F S Pedersen.   

Abstract

cis-Acting transcriptional control elements in the U3 region of the murine retrovirus Akv were analyzed in mouse NIH 3T3 fibroblast cells by using a transient expression vector system based upon a complete long terminal repeat with linked flanking sequences. Deletion analysis pointed to the essential role of sequences within the 99-base-pair direct repeats, and a fragment encompassing the two repeats was found to possess orientation-independent enhancer activity when positioned either upstream or downstream of the transcriptional unit. Removal of one copy of the 99-base-pair repeat led to a reduction in activity of about 2.5-fold when located in an intact U3 environment but to reductions of up to 2 orders of magnitude when placed in other sequence contexts. Our studies of enhancer functions in the presence of one versus two copies of the tandem repeat point to duplicate functions of repeat sequences and sequences flanking the repeat region and emphasize the complex overall organization of this U3 region.

Entities:  

Mesh：

Year:  1990        PMID： 2161937      PMCID： PMC249523          DOI： 10.1128/JVI.64.7.3185-3191.1990

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  45 in total

1.  Multiple sequence elements in the U3 region of the leukemogenic murine retrovirus SL3-2 contribute to cell-dependent gene expression.

Authors:  H Y Dai; M Etzerodt; A J Baekgaard; S Lovmand; P Jørgensen; N O Kjeldgaard; F S Pedersen
Journal:  Virology       Date:  1990-04       Impact factor: 3.616

2.  Nucleotide sequences of the retroviral long terminal repeats and their adjacent regions.

Authors:  H R Chen; W C Barker
Journal:  Nucleic Acids Res       Date:  1984-02-24       Impact factor: 16.971

3.  Nucleotide sequence of AKV murine leukemia virus.

Authors:  W Herr
Journal:  J Virol       Date:  1984-02       Impact factor: 5.103

4.  Characterization of enhancer elements in the long terminal repeat of Moloney murine sarcoma virus.

Authors:  L A Laimins; P Gruss; R Pozzatti; G Khoury
Journal:  J Virol       Date:  1984-01       Impact factor: 5.103

5.  Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat.

Authors:  J Lenz; D Celander; R L Crowther; R Patarca; D W Perkins; W A Haseltine
Journal:  Nature       Date:  1984 Mar 29-Apr 4       Impact factor: 49.962

6.  Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.

Authors:  X Soberon; L Covarrubias; F Bolivar
Journal:  Gene       Date:  1980-05       Impact factor: 3.688

7.  The high leukemogenic potential of Gross passage A murine leukemia virus maps in the region of the genome corresponding to the long terminal repeat and to the 3' end of env.

Authors:  L DesGroseillers; R Villemur; P Jolicoeur
Journal:  J Virol       Date:  1983-07       Impact factor: 5.103

8.  Analysis of FBJ-MuSV provirus and c-fos (mouse) gene reveals that viral and cellular fos gene products have different carboxy termini.

Authors:  C Van Beveren; F van Straaten; T Curran; R Müller; I M Verma
Journal:  Cell       Date:  1983-04       Impact factor: 41.582

9.  Molecular cloning of viral DNA from leukemogenic Gross passage A murine leukemia virus and nucleotide sequence of its long terminal repeat.

Authors:  R Villemur; E Rassart; L DesGroseillers; P Jolicoeur
Journal:  J Virol       Date:  1983-02       Impact factor: 5.103

10.  Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells.

Authors:  C V Hall; P E Jacob; G M Ringold; F Lee
Journal:  J Mol Appl Genet       Date:  1983
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  19 in total

1.  Transformation of a novel direct-repeat repressor element into a promoter and enhancer by multimerisation.

Authors:  K C Chang; E Hansen; T Jaenicke; G Goldspink; P Butterworth
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

2.  Contributions to transcriptional activity and to viral leukemogenicity made by sequences within and downstream of the MCF13 murine leukemia virus enhancer.

Authors:  J C Tupper; H Chen; E F Hays; G C Bristol; F K Yoshimura
Journal:  J Virol       Date:  1992-12       Impact factor: 5.103

3.  Analysis of the significance of two single-base-pair differences in the SL3-3 and Akv virus long terminal repeats.

Authors:  H L Morrison; H Y Dai; F S Pedersen; J Lenz
Journal:  J Virol       Date:  1991-02       Impact factor: 5.103

4.  A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses.

Authors:  M Duch; K Paludan; J Lovmand; L Pedersen; P Jørgensen; F S Pedersen
Journal:  Nucleic Acids Res       Date:  1993-10-11       Impact factor: 16.971

5.  Mapping of a major osteomagenic determinant of murine leukemia virus RFB-14 to non-long terminal repeat sequences.

Authors:  M Ostergaard; L Pedersen; J Schmidt; A Luz; J Lovmand; V Erfle; F S Pedersen; P G Strauss
Journal:  J Virol       Date:  1997-01       Impact factor: 5.103

6.  Duplication of U3 sequences in the long terminal repeat of mink cell focus-inducing viruses generates redundancies of transcription factor binding sites important for the induction of thymomas.

Authors:  Nancy L DiFronzo; Marisa Frieder; Scott A Loiler; Quynh N Pham; Christie A Holland
Journal:  J Virol       Date:  2003-03       Impact factor: 5.103

7.  E- vectors: development of novel self-inactivating and self-activating retroviral vectors for safer gene therapy.

Authors:  J G Julias; D Hash; V K Pathak
Journal:  J Virol       Date:  1995-11       Impact factor: 5.103

8.  B-Cell lymphoma induction by akv murine leukemia viruses harboring one or both copies of the tandem repeat in the U3 enhancer.

Authors:  J Lovmand; A B Sorensen; J Schmidt; M Ostergaard; A Luz; F S Pedersen
Journal:  J Virol       Date:  1998-07       Impact factor: 5.103

9.  Basic helix-loop-helix proteins in murine type C retrovirus transcriptional regulation.

Authors:  A L Nielsen; N Pallisgaard; F S Pedersen; P Jørgensen
Journal:  J Virol       Date:  1994-09       Impact factor: 5.103

10.  Murine helix-loop-helix transcriptional activator proteins binding to the E-box motif of the Akv murine leukemia virus enhancer identified by cDNA cloning.

Authors:  A L Nielsen; N Pallisgaard; F S Pedersen; P Jørgensen
Journal:  Mol Cell Biol       Date:  1992-08       Impact factor: 4.272
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