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Literature DB >> 2159637

Backbone-modified oligonucleotides containing a butanediol-1,3 moiety as a 'vicarious segment' for the deoxyribosyl moiety--synthesis and enzyme studies.

A Wilk¹, M Koziolkiewicz, A Grajkowski, B Uznanski, W J Stec.

Abstract

Sequential single replacement of nucleosides within the decanucleotide d[GGGAATTCCC] (7) by means of a butanediol-1,3 residue allowed us to obtain a set of ten decanucleotides containing 'vicarious' (V) carbon-phosphate fragments. These analogues were further used as substrates for svPDE, nuclease PI and EcoR1 endonuclease. Interestingly, replacement of any of the nucleosides within the canonical sequence ...GAATTC... by the butanediol-1,3 'vicarious' segment discriminates such constructs 8c-h as substrates for Eco-R1 enzyme.

Entities: Chemical

Mesh：

Substances：

Year: 1990 PMID： 2159637 PMCID： PMC330684 DOI： 10.1093/nar/18.8.2065

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

5 in total

1. Synthesis of thymidine oligonucleotides by phosphite triester intermediates.

Authors: R L Letsinger; W B Lunsford
Journal: J Am Chem Soc Date: 1976-06-09 Impact factor: 15.419

2. A simple, general procedure for purifying restriction endonucleases.

Authors: T A Bickle; V Pirrotta; R Imber
Journal: Nucleic Acids Res Date: 1977-08 Impact factor: 16.971

3. Type II restriction endonucleases cleave single-stranded DNAs in general.

Authors: K Nishigaki; Y Kaneko; H Wakuda; Y Husimi; T Tanaka
Journal: Nucleic Acids Res Date: 1985-08-26 Impact factor: 16.971

4. Cleavage of single stranded oligonucleotides by EcoRI restriction endonuclease.

Authors: N Bischofberger; P G Ng; T R Webb; M D Matteucci
Journal: Nucleic Acids Res Date: 1987-01-26 Impact factor: 16.971

5. Reversed-phase high-performance liquid chromatographic separation of diastereomeric phosphorothioate analogues of oligodeoxyribonucleotides and other backbone-modified congeners of DNA.

Authors: W J Stec; G Zon; B Uznanski
Journal: J Chromatogr Date: 1985-06-19

5 in total

4 in total

1. Cleavage of synthetic substrates containing non-nucleotide inserts by restriction endonucleases. Change in the cleavage specificity of endonuclease SsoII.

Authors: E A Kubareva; O V Petrauskene; A S Karyagina; V N Tashlitsky; I I Nikolskaya; E S Gromova
Journal: Nucleic Acids Res Date: 1992-09-11 Impact factor: 16.971