| Literature DB >> 21565264 |
Thomas J Hoffmann1, Mark N Kvale, Stephanie E Hesselson, Yiping Zhan, Christine Aquino, Yang Cao, Simon Cawley, Elaine Chung, Sheryl Connell, Jasmin Eshragh, Marcia Ewing, Jeremy Gollub, Mary Henderson, Earl Hubbell, Carlos Iribarren, Jay Kaufman, Richard Z Lao, Yontao Lu, Dana Ludwig, Gurpreet K Mathauda, William McGuire, Gangwu Mei, Sunita Miles, Matthew M Purdy, Charles Quesenberry, Dilrini Ranatunga, Sarah Rowell, Marianne Sadler, Michael H Shapero, Ling Shen, Tanushree R Shenoy, David Smethurst, Stephen K Van den Eeden, Larry Walter, Eunice Wan, Reid Wearley, Teresa Webster, Christopher C Wen, Li Weng, Rachel A Whitmer, Alan Williams, Simon C Wong, Chia Zau, Andrea Finn, Catherine Schaefer, Pui-Yan Kwok, Neil Risch.
Abstract
The success of genome-wide association studies has paralleled the development of efficient genotyping technologies. We describe the development of a next-generation microarray based on the new highly-efficient Affymetrix Axiom genotyping technology that we are using to genotype individuals of European ancestry from the Kaiser Permanente Research Program on Genes, Environment and Health (RPGEH). The array contains 674,517 SNPs, and provides excellent genome-wide as well as gene-based and candidate-SNP coverage. Coverage was calculated using an approach based on imputation and cross validation. Preliminary results for the first 80,301 saliva-derived DNA samples from the RPGEH demonstrate very high quality genotypes, with sample success rates above 94% and over 98% of successful samples having SNP call rates exceeding 98%. At steady state, we have produced 462 million genotypes per week for each Axiom system. The new array provides a valuable addition to the repertoire of tools for large scale genome-wide association studies.Entities:
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Year: 2011 PMID： 21565264 PMCID： PMC3146553 DOI： 10.1016/j.ygeno.2011.04.005
Source DB: PubMed Journal: Genomics ISSN： 0888-7543 Impact factor: 5.736