| Literature DB >> 21526835 |
Julie B Rannes1, Avgousta Ioannou, Simon C Willies, Gideon Grogan, Carsten Behrens, Sabine L Flitsch, Nicholas J Turner.
Abstract
A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans D-mannose (Man) and D-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface.Entities:
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Year: 2011 PMID： 21526835 DOI： 10.1021/ja2018477
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419