OBJECTIVES: Investigate the use of optical reflectance spectroscopy to differentiate malignant and nonmalignant tissues in head and neck lesions and characterize corresponding oxygen tissue biomarkers that are associated with pathologic diagnosis. STUDY DESIGN: Cross-sectional study. SETTING: Tertiary Veterans Administration Medical Center. SUBJECTS AND METHODS: All patients undergoing panendoscopy with biopsy for suspected head and neck cancer were eligible. Prior to taking tissue samples, the optical probe was placed at 3 locations to collect diffuse reflectance data. These locations were labeled "tumor," "immediately adjacent," and "distant normal tissue." Biopsies were taken of each of these respective sites. The diffuse reflectance spectra were analyzed, and biomarker-specific absorption data were extracted using an inverse Monte Carlo algorithm for malignant and nonmalignant tissues. Histopathological analysis was performed and used as the gold standard to analyze the optical biomarker data. RESULTS: Twenty-one patients with mucosal squamous cell carcinoma of the head and neck were identified and selected to participate in the study. Statistically significant differences in oxygen saturation (P = .001) and oxygenated hemoglobin (P = .019) were identified between malignant and nonmalignant tissues. CONCLUSION: This study established proof of principle that optical spectroscopy can be used in the head and neck areas to detect malignant tissue. Furthermore, tissue biomarkers were correlated with a diagnosis of malignancy.
OBJECTIVES: Investigate the use of optical reflectance spectroscopy to differentiate malignant and nonmalignant tissues in head and neck lesions and characterize corresponding oxygen tissue biomarkers that are associated with pathologic diagnosis. STUDY DESIGN: Cross-sectional study. SETTING: Tertiary Veterans Administration Medical Center. SUBJECTS AND METHODS: All patients undergoing panendoscopy with biopsy for suspected head and neck cancer were eligible. Prior to taking tissue samples, the optical probe was placed at 3 locations to collect diffuse reflectance data. These locations were labeled "tumor," "immediately adjacent," and "distant normal tissue." Biopsies were taken of each of these respective sites. The diffuse reflectance spectra were analyzed, and biomarker-specific absorption data were extracted using an inverse Monte Carlo algorithm for malignant and nonmalignant tissues. Histopathological analysis was performed and used as the gold standard to analyze the optical biomarker data. RESULTS: Twenty-one patients with mucosal squamous cell carcinoma of the head and neck were identified and selected to participate in the study. Statistically significant differences in oxygen saturation (P = .001) and oxygenated hemoglobin (P = .019) were identified between malignant and nonmalignant tissues. CONCLUSION: This study established proof of principle that optical spectroscopy can be used in the head and neck areas to detect malignant tissue. Furthermore, tissue biomarkers were correlated with a diagnosis of malignancy.
Authors: Richard A Schwarz; Wen Gao; Crystal Redden Weber; Cristina Kurachi; J Jack Lee; Adel K El-Naggar; Rebecca Richards-Kortum; Ann M Gillenwater Journal: Cancer Date: 2009-04-15 Impact factor: 6.860
Authors: J Quincy Brown; Lee G Wilke; Joseph Geradts; Stephanie A Kennedy; Gregory M Palmer; Nirmala Ramanujam Journal: Cancer Res Date: 2009-03-17 Impact factor: 12.701
Authors: Fangyao Hu; Karthik Vishwanath; H Wolfgang Beumer; Liana Puscas; Hamid R Afshari; Ramon M Esclamado; Richard Scher; Samuel Fisher; Justin Lo; Christine Mulvey; Nirmala Ramanujam; Walter T Lee Journal: Oral Oncol Date: 2014-07-16 Impact factor: 5.337