Literature DB >> 214681

Application of a solid-phase radioimmunoassay and immune electron microscopy for hepatitis A in diagnosis and research.

B Flehmig, M Ranke, H Frank, H J Gerth.   

Abstract

With crude virus suspensions from stool and antibodies from hepatitis-A patients, a solid-phase radioimmunoassay (RIA) for detection of hepatitis virus A (HVA) had antibodies against hepatitis virus A (anti-HVA) has been developed. Examples for the application of this test are demonstrated. Virus particles from the stools of the two patients were further characterized. Serologically, they were identical or very similar to the MS-1 strain. Isopycnic CsCl-gradient centrifugation of both strains revealed two peaks, but the particles of different densities did not differ in size or serologically. A modification of the RIA was also useful for determination of IgM antibodies in patients' sera fractionated by sucrose-density centrifugation. The application of the RIA method for serologic epidemiology is demonstrated by a comparison of anti-HVA prevalence in German and non-German women residing in Germany.

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Year:  1978        PMID： 214681     DOI： 10.1007/bf02121149

Source DB:  PubMed          Journal:  Med Microbiol Immunol        ISSN： 0300-8584            Impact factor:   3.402


  15 in total

1.  CsCl banding of hepatitis A-associated virus-like particles.

Authors:  D W Bradley; C L Hornbeck; E H Cood; J E Maynard; C R Gravelle
Journal:  J Infect Dis       Date:  1975-03       Impact factor: 5.226

2.  A specific complement-fixation test for human hepatitis a employing CR326 virus antigen. Diagnosis and epidemiology.

Authors:  P J Provost; O L Ittensohn; V M Villarejos; M R Hilleman
Journal:  Proc Soc Exp Biol Med       Date:  1975-04

3.  Similarities of two hepatitis A virus strains.

Authors:  J Rakela; O H Fay; D Stevenson; I Gordon; J W Mosley
Journal:  Bull World Health Organ       Date:  1976       Impact factor: 9.408

4.  Preliminary serologic studies of antibody to hepatitis A virus in populations in the United States.

Authors:  J E Maynard; D W Bradley; C L Hornbeck; R M Fields; I L Doto; F B Hollinger
Journal:  J Infect Dis       Date:  1976-11       Impact factor: 5.226

5.  Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies.

Authors:  T Vesikari; A Vaheri
Journal:  Br Med J       Date:  1968-01-27

6.  The relationship between a 27-nm virus-like particle and hepatitis A as demonstrated by immune electron microscopy.

Authors:  S A Locarnini; A A Ferris; A C Stott; I D Gust
Journal:  Intervirology       Date:  1974       Impact factor: 1.763

7.  Hepatitis A: report of a common-source outbreak with recovery of a possible etiologic agent. II. Laboratory studies.

Authors:  C R Gravelle; C L Hornbeck; J E Maynard; C A Schable; E H Cook; D W Bradley
Journal:  J Infect Dis       Date:  1975-02       Impact factor: 5.226

8.  Serodiagnosis of viral hepatitis A: detection of acute-phase immunoglobulin M anti-hepatitis A virus by radioimmunoassay.

Authors:  D W Bradley; J E Maynard; S H Hindman; C L Hornbeck; H A Fields; K A McCaustland; E H Cook
Journal:  J Clin Microbiol       Date:  1977-05       Impact factor: 5.948

9.  [Frequency of hepatitis A antibodies in populations of various European countries].

Authors:  G G Frösner; H R Frösner; H Haas; K Dietz; U Sugg; W Schneider
Journal:  Schweiz Med Wochenschr       Date:  1977-02-05

10.  Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness.

Authors:  S M Feinstone; A Z Kapikian; R H Purceli
Journal:  Science       Date:  1973-12-07       Impact factor: 47.728
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  8 in total

1.  Myelopoiesis in vitro is suppressed by hepatitis A virus.

Authors:  F W Busch; A Kunst; B Flehmig; H G Mergenthaler; G Pawelec; A Vallbracht
Journal:  Ann Hematol       Date:  1992-06       Impact factor: 3.673

2.  Hepatitis A-virus in cell culture. IV. Comparison of cell-culture-produced HAV with stool-derived HAV in diagnostic test systems.

Authors:  B Flehmig; M Pfisterer; A Vallbracht
Journal:  Med Microbiol Immunol       Date:  1984       Impact factor: 3.402

3.  Comparison of ozone inactivation, in flowing water, of hepatitis A virus, poliovirus 1, and indicator organisms.

Authors:  K Herbold; B Flehmig; K Botzenhart
Journal:  Appl Environ Microbiol       Date:  1989-11       Impact factor: 4.792

4.  Hepatitis A-virus in cell culture. V. Neutralizing antibodies against hepatitis A-virus.

Authors:  J Zahn; A Vallbracht; B Flehmig
Journal:  Med Microbiol Immunol       Date:  1984       Impact factor: 3.402

5.  Immunopotentiating reconstituted influenza virus virosome vaccine delivery system for immunization against hepatitis A.

Authors:  R Glück; R Mischler; S Brantschen; M Just; B Althaus; S J Cryz
Journal:  J Clin Invest       Date:  1992-12       Impact factor: 14.808

6.  Hepatitis A-virus in cell culture: I. propagation of different hepatitis A-virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4).

Authors:  B Flehmig
Journal:  Med Microbiol Immunol       Date:  1980       Impact factor: 3.402

7.  Nucleotide sequence of wild-type hepatitis A virus GBM in comparison with two cell culture-adapted variants.

Authors:  J Graff; A Normann; S M Feinstone; B Flehmig
Journal:  J Virol       Date:  1994-01       Impact factor: 5.103

8.  Hepatitis A virus in cell culture. II. Growth characteristics of hepatitis A virus in Frhk-4/R cells.

Authors:  B Flehmig
Journal:  Med Microbiol Immunol       Date:  1981       Impact factor: 3.402
  8 in total

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