Literature DB >> 6325856

Hepatitis A-virus in cell culture. IV. Comparison of cell-culture-produced HAV with stool-derived HAV in diagnostic test systems.

B Flehmig, M Pfisterer, A Vallbracht.   

Abstract

Hepatitis A-virus produced in Frhk-4/R cells is used and compared with stool-derived HAV as an antigen in diagnostic test systems. It is shown that the two antigens react identically in the anti-HAV test with anti-HAV-IgG positive human sera. In the anti-HAV-IgM test, too, stool-derived HAV and cell-culture-produced HAV react very similarly. The titres of anti-HAV-IgG and anti-HAV-IgM positive sera, obtained with stool and cell-culture HAV in quantitative antibody determinations, are shown to be identical. It is shown that the problem of HAV antigen production can now be solved by propagation of HAV with Frhk-4/R cells and that this antigen is very useful in diagnostic test systems.

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Year:  1984        PMID： 6325856     DOI： 10.1007/bf02123715

Source DB:  PubMed          Journal:  Med Microbiol Immunol        ISSN： 0300-8584            Impact factor:   3.402


  15 in total

1.  Propagation of human hepatitis A virus in cell culture in vitro.

Authors:  P J Provost; M R Hilleman
Journal:  Proc Soc Exp Biol Med       Date:  1979-02

2.  Application of a solid-phase radioimmunoassay and immune electron microscopy for hepatitis A in diagnosis and research.

Authors:  B Flehmig; M Ranke; H Frank; H J Gerth
Journal:  Med Microbiol Immunol       Date:  1978-11-17       Impact factor: 3.402

3.  Polyomavirus in fetal rhesus monkey kidney cell lines used to grow hepatitis A virus.

Authors:  J V Parry; J E Richmond; S D Gardner
Journal:  Lancet       Date:  1983-04-30       Impact factor: 79.321

4.  A microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody.

Authors:  R H Purcell; D C Wong; Y Moritsugu; J L Dienstag; J A Routenberg; J D Boggs
Journal:  J Immunol       Date:  1976-02       Impact factor: 5.422

5.  A solid-phase radioimmunoassay for detection of IgM antibodies to hepatitis A virus.

Authors:  B Flehmig; M Ranke; H Berthold; H J Gerth
Journal:  J Infect Dis       Date:  1979-08       Impact factor: 5.226

6.  Hepatitis A-virus in cell culture: I. propagation of different hepatitis A-virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4).

Authors:  B Flehmig
Journal:  Med Microbiol Immunol       Date:  1980       Impact factor: 3.402

7.  Serodiagnosis of viral hepatitis A: detection of acute-phase immunoglobulin M anti-hepatitis A virus by radioimmunoassay.

Authors:  D W Bradley; J E Maynard; S H Hindman; C L Hornbeck; H A Fields; K A McCaustland; E H Cook
Journal:  J Clin Microbiol       Date:  1977-05       Impact factor: 5.948

8.  Detection of hepatitis A virus in the feces of patients with naturally acquired infections.

Authors:  A G Coulepis; S A Locarnini; N I Lehmann; I D Gust
Journal:  J Infect Dis       Date:  1980-02       Impact factor: 5.226

9.  Hepatitis A virus in cell culture. III. Propagation of hepatitis A virus in human embryo kidney cells and human embryo fibroblast strains.

Authors:  B Flehmig; A Vallbracht; G Wurster
Journal:  Med Microbiol Immunol       Date:  1981       Impact factor: 3.402

10.  Hepatitis A virus in cell culture. II. Growth characteristics of hepatitis A virus in Frhk-4/R cells.

Authors:  B Flehmig
Journal:  Med Microbiol Immunol       Date:  1981       Impact factor: 3.402
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