Literature DB >> 6088963

Hepatitis A-virus in cell culture. V. Neutralizing antibodies against hepatitis A-virus.

J Zahn, A Vallbracht, B Flehmig.   

Abstract

A test system for the detection of neutralizing antibodies against hepatitis A-virus (anti-HAV-Nt) is presented. The anti-HAV-Nt assay is performed with Frhk-4/R cells and the hepatitis A virus (HAV) strain GBM/Frhk-4/R which has been adapted to these cells. Non-neutralized HAV is demonstrated 14 days after infection of Frhk-4/R cells using a radio-immunoassay for detecting newly grown HAV. The influence of differing amounts of HAV on the anti-HAV-Nt titre and the effect of variations in incubation time of virus-serum mixtures are described. The time course of anti-HAV-Nt is shown in sera from a hepatitis-A patient which were taken at different stages of the disease. Anti-HAV-Nt is compared with anti-HAV and anti-HAV-IgM. It is shown that anti-HAV-Nt correlates closely with anti-HAV and separated anti-HAV-IgG, but only slightly with anti-HAV-IgM. The test system presented makes possible the demonstration of neutralizing antibodies against HAV in gamma-globulin preparations and during vaccination studies.

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Year:  1984        PMID： 6088963     DOI： 10.1007/bf02123564

Source DB:  PubMed          Journal:  Med Microbiol Immunol        ISSN： 0300-8584            Impact factor:   3.402


  20 in total

1.  A specific complement-fixation test for human hepatitis a employing CR326 virus antigen. Diagnosis and epidemiology.

Authors:  P J Provost; O L Ittensohn; V M Villarejos; M R Hilleman
Journal:  Proc Soc Exp Biol Med       Date:  1975-04

2.  The antibody response following hepatitis A infection.

Authors:  S A Locarnini; A A Ferris; N I Lehmann; I D Gust
Journal:  Intervirology       Date:  1977       Impact factor: 1.763

3.  Application of a solid-phase radioimmunoassay and immune electron microscopy for hepatitis A in diagnosis and research.

Authors:  B Flehmig; M Ranke; H Frank; H J Gerth
Journal:  Med Microbiol Immunol       Date:  1978-11-17       Impact factor: 3.402

4.  Experience at the National Communicable Disease Center with micro serological techniques for the diagnosis of viral diseases.

Authors:  H L Casey
Journal:  Health Lab Sci       Date:  1970-10

5.  Microneutralization test for the reoviruses. Application to detection and assay of antibodies in sera of laboratory animals.

Authors:  N J Schmidt; E H Lennette; M F Hanahoe
Journal:  Proc Soc Exp Biol Med       Date:  1966-04

6.  A solid-phase radioimmunoassay for detection of IgM antibodies to hepatitis A virus.

Authors:  B Flehmig; M Ranke; H Berthold; H J Gerth
Journal:  J Infect Dis       Date:  1979-08       Impact factor: 5.226

7.  Hepatitis A-virus in cell culture: I. propagation of different hepatitis A-virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4).

Authors:  B Flehmig
Journal:  Med Microbiol Immunol       Date:  1980       Impact factor: 3.402

8.  Coproantibodies in hepatitis A: detection by enzyme-linked immunosorbent assay and immune electron microscopy.

Authors:  S A Locarnini; A G Coulepis; J Kaldor; I D Gust
Journal:  J Clin Microbiol       Date:  1980-06       Impact factor: 5.948

9.  Serodiagnosis of viral hepatitis A: detection of acute-phase immunoglobulin M anti-hepatitis A virus by radioimmunoassay.

Authors:  D W Bradley; J E Maynard; S H Hindman; C L Hornbeck; H A Fields; K A McCaustland; E H Cook
Journal:  J Clin Microbiol       Date:  1977-05       Impact factor: 5.948

10.  Hepatitis A virus in cell culture. II. Growth characteristics of hepatitis A virus in Frhk-4/R cells.

Authors:  B Flehmig
Journal:  Med Microbiol Immunol       Date:  1981       Impact factor: 3.402
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  3 in total

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Review 2.  Innate and adaptive immune responses against picornaviruses and their counteractions: An overview.

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Journal:  World J Virol       Date:  2012-06-12

Review 3.  Adaptive Immune Responses in Hepatitis A Virus and Hepatitis E Virus Infections.

Authors:  Christopher M Walker
Journal:  Cold Spring Harb Perspect Med       Date:  2019-09-03       Impact factor: 6.915
  3 in total

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