Literature DB >> 6266620

Neonatal bovine enteritis: detection of rotavirus by counterimmunoelectrophoresis and enzyme-linked immunosorbent assay.

L J Ekern, I A Schipper, K J McMahon.   

Abstract

Counterimmunoelectrophoresis and enzyme-linked immunosorbent assay specific for rotavirus were performed on 253 calf gut and fecal specimens from eastern North Dakota. Rotavirus antigen was detected in 29 specimens by counterimmunoelectrophoresis and in 52 specimens by enzyme-linked immunosorbent assay. All of the counterimmunoelectrophoresis-positive specimens were positive by enzyme-linked immunosorbent assay. The counterimmunoelectrophoresis and the enzyme-linked immunosorbent assay, using a rabbit antirotavirus antibodies produced a golf ball-induced granuloma, were rapid and specific tests for the detection of rotavirus.

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Year:  1981        PMID： 6266620      PMCID： PMC1320140     

Source DB:  PubMed          Journal:  Can J Comp Med        ISSN： 0008-4050


  15 in total

1.  Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves.

Authors:  D J Ellens; P W de Leeuw
Journal:  J Clin Microbiol       Date:  1977-11       Impact factor: 5.948

2.  Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species.

Authors:  R H Yolken; B Barbour; R G Wyatt; A R Kalica; A Z Kapikian; R M Chanock
Journal:  Science       Date:  1978-07-21       Impact factor: 47.728

3.  Rotavirus isolation and cultivation in the presence of trypsin.

Authors:  L A Babiuk; K Mohammed; L Spence; M Fauvel; R Petro
Journal:  J Clin Microbiol       Date:  1977-12       Impact factor: 5.948

4.  Hemagglutination by simian rotavirus.

Authors:  A R Kalica; J D James; A Z Kapikian
Journal:  J Clin Microbiol       Date:  1978-03       Impact factor: 5.948

5.  Serotyping herpes simplex virus isolated by enzyme-linked immunosorbent assays.

Authors:  K W Mills; E H Gerlach; J W Bell; M E Farkas; R J Taylor
Journal:  J Clin Microbiol       Date:  1978-01       Impact factor: 5.948

6.  Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces.

Authors:  D J Ellens; P W de Leeuw; P J Straver; J A van Balken
Journal:  Med Microbiol Immunol       Date:  1978-11-17       Impact factor: 3.402

7.  Evaluation of some of the parameters of the enzyme-linked immunospecific assay.

Authors:  S L Bullock; K W Walls
Journal:  J Infect Dis       Date:  1977-10       Impact factor: 5.226

8.  A microtiter solid phase radioimmunoassay for detection of the human reovirus-like agent in stools.

Authors:  A R Kalica; R H Purcell; M M Sereno; R G Wyatt; H W Kim; R M Chanock; A Z Kapikian
Journal:  J Immunol       Date:  1977-04       Impact factor: 5.422

9.  Diagnosis of rotavirus infection by cell culture.

Authors:  A S Bryden; H A Davies; M E Thouless; T H Flewitt
Journal:  J Med Microbiol       Date:  1977-02       Impact factor: 2.472

10.  Rapid diagnosis of rotavirus infections: comparison of electron microscopy and immunoelectroosmophoresis for the detection of rotavirus in human infantile gastroenteritis.

Authors:  P C Grauballe; J Genner; A Meyling; A Hornsleth
Journal:  J Gen Virol       Date:  1977-05       Impact factor: 3.891
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  1 in total

1.  Characterization of the coliform and enteric bacilli in the environment of calves with colibacillosis.

Authors:  P I Plews; M C Bromel; I A Schipper
Journal:  Appl Environ Microbiol       Date:  1985-04       Impact factor: 4.792
  1 in total

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