Literature DB >> 2146161

The specific incorporation of labelled aromatic amino acids into proteins through growth of bacteria in the presence of glyphosate. Application to fluorotryptophan labelling to the H(+)-ATPase of Escherichia coli and NMR studies.

H W Kim1, J A Perez, S J Ferguson, I D Campbell.   

Abstract

Growth of Escherichia coli in the presence of glyphosate, an inhibitor of aromatic amino acid biosynthesis, has permitted the production of proton translocating ATPase that is specifically labelled with 5-fluorotryptophan. Five sets of 19F nuclear magnetic resonances are resolved. The use of glyphosate should be of wide applicability in the preparation of proteins labelled in aromatic amino acid residues for NMR studies.

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Year:  1990        PMID： 2146161     DOI： 10.1016/0014-5793(90)80442-l

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  20 in total

1.  Cell-free synthesis of membrane subunits of ATP synthase in phospholipid bicelles: NMR shows subunit a fold similar to the protein in the cell membrane.

Authors:  Eva-Maria E Uhlemann; Hannah E Pierson; Robert H Fillingame; Oleg Y Dmitriev
Journal:  Protein Sci       Date:  2012-01-04       Impact factor: 6.725

2.  Structural studies of Bcl-xL/ligand complexes using 19F NMR.

Authors:  Liping Yu; Philip J Hajduk; Jamey Mack; Edward T Olejniczak
Journal:  J Biomol NMR       Date:  2006-04       Impact factor: 2.835

Review 3.  Use of 19F NMR to probe protein structure and conformational changes.

Authors:  M A Danielson; J J Falke
Journal:  Annu Rev Biophys Biomol Struct       Date:  1996

4.  Protein-observed (19)F-NMR for fragment screening, affinity quantification and druggability assessment.

Authors:  Clifford T Gee; Keith E Arntson; Andrew K Urick; Neeraj K Mishra; Laura M L Hawk; Andrea J Wisniewski; William C K Pomerantz
Journal:  Nat Protoc       Date:  2016-07-14       Impact factor: 13.491

5.  High-Sensitivity Detection of Nanometer 1H-19F Distances for Protein Structure Determination by 1H-Detected Fast MAS NMR.

Authors:  Alexander A Shcherbakov; Venkata Shiva Mandala; Mei Hong
Journal:  J Phys Chem B       Date:  2019-05-08       Impact factor: 2.991

6.  Structure, dynamics, and ionization equilibria of the tyrosine residues in Bacillus circulans xylanase.

Authors:  Simon J Baturin; Mark Okon; Lawrence P McIntosh
Journal:  J Biomol NMR       Date:  2011-09-13       Impact factor: 2.835

7.  Using O2 to probe membrane immersion depth by 19F NMR.

Authors:  R S Prosser; P A Luchette; P W Westerman
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-29       Impact factor: 11.205

8.  Auto-induction medium containing glyphosate for high-level incorporation of unusual aromatic amino acids into proteins.

Authors:  Muniasamy Neerathilingam; John L Markley
Journal:  Biotechniques       Date:  2010-09       Impact factor: 1.993

9.  (19)F NMR reveals multiple conformations at the dimer interface of the nonstructural protein 1 effector domain from influenza A virus.

Authors:  James M Aramini; Keith Hamilton; Li-Chung Ma; G V T Swapna; Paul G Leonard; John E Ladbury; Robert M Krug; Gaetano T Montelione
Journal:  Structure       Date:  2014-02-27       Impact factor: 5.006

10.  Attractant- and disulfide-induced conformational changes in the ligand binding domain of the chemotaxis aspartate receptor: a 19F NMR study.

Authors:  M A Danielson; H P Biemann; D E Koshland; J J Falke
Journal:  Biochemistry       Date:  1994-05-24       Impact factor: 3.162
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