Literature DB >> 21450967

Modifications of commercial toxigenic Clostridium difficile PCR resulting in improved economy and workflow efficiency.

Erik Munson1, Dorothy Bilbo, Mary Paul, Maureen Napierala, Jeanne E Hryciuk.   

Abstract

Expense inherent to molecular diagnostics may prevent laboratories from utilizing real-time PCR for Clostridium difficile infection. Frozen master mix and overnight aliquot modifications of the BD GeneOhm Cdiff assay failed to impact performance indices compared to the package insert protocol (P ≥ 0.31), provided accurate results, and decreased reagent expenditure.

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Year:  2011        PMID： 21450967      PMCID： PMC3122720          DOI： 10.1128/JCM.00261-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Simplified PCR set-up using a frozen preformulated mix for the detection of cytomegalovirus.

Authors:  B Kofler; A Klausegger
Journal:  Diagn Microbiol Infect Dis       Date:  1999-05       Impact factor: 2.803

2.  Freezing complete polymerase chain reaction master mix reagents for routine molecular diagnostics.

Authors:  Donna Marie West; Jason Sawyer
Journal:  J Vet Diagn Invest       Date:  2006-11       Impact factor: 1.279

3.  Cost-effective modification of a commercial PCR assay for detection of methicillin-resistant or -susceptible Staphylococcus aureus in positive blood cultures.

Authors:  Erik Munson; Timothy Kramme; Anne Culver; Jeanne E Hryciuk; Ronald F Schell
Journal:  J Clin Microbiol       Date:  2010-02-10       Impact factor: 5.948

4.  Evaluation of the C.Diff Quik Chek Complete Assay, a new glutamate dehydrogenase and A/B toxin combination lateral flow assay for use in rapid, simple diagnosis of clostridium difficile disease.

Authors:  Susan E Sharp; Lila O Ruden; Julie C Pohl; Patricia A Hatcher; Linda M Jayne; W Michael Ivie
Journal:  J Clin Microbiol       Date:  2010-04-07       Impact factor: 5.948

5.  Cost analysis of a hospital-wide selective screening programme for methicillin-resistant Staphylococcus aureus (MRSA) carriers in the context of diagnosis related groups (DRG) payment.

Authors:  M H Wernitz; S Keck; S Swidsinski; S Schulz; S K Veit
Journal:  Clin Microbiol Infect       Date:  2005-06       Impact factor: 8.067

6.  Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.

Authors:  L Monteiro; D Bonnemaison; A Vekris; K G Petry; J Bonnet; R Vidal; J Cabrita; F Mégraud
Journal:  J Clin Microbiol       Date:  1997-04       Impact factor: 5.948

7.  Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples.

Authors:  Paul D Stamper; Romina Alcabasa; Deborah Aird; Wisal Babiker; Jennifer Wehrlin; Ijeoma Ikpeama; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2008-12-10       Impact factor: 5.948

8.  Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile.

Authors:  Megan E Reller; Clara A Lema; Trish M Perl; Mian Cai; Tracy L Ross; Kathleen A Speck; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2007-09-05       Impact factor: 5.948

9.  Comparison of real-time PCR for detection of the tcdC gene with four toxin immunoassays and culture in diagnosis of Clostridium difficile infection.

Authors:  Lynne M Sloan; Brian J Duresko; Daniel R Gustafson; Jon E Rosenblatt
Journal:  J Clin Microbiol       Date:  2008-04-23       Impact factor: 5.948

10.  Is a two-step glutamate dehyrogenase antigen-cytotoxicity neutralization assay algorithm superior to the premier toxin A and B enzyme immunoassay for laboratory detection of Clostridium difficile?

Authors:  Peter H Gilligan
Journal:  J Clin Microbiol       Date:  2008-02-06       Impact factor: 5.948
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  3 in total

1.  Frozen master mix-based modification of commercial reverse transcriptase PCR for detection of influenza and respiratory syncytial viruses.

Authors:  Brooke Wenninger; Erik Munson; Maureen Napierala; Kimber L Munson; Dorothy Bilbo; Jeanne E Hryciuk
Journal:  J Clin Microbiol       Date:  2015-01-28       Impact factor: 5.948

2.  Application of Alternative Nucleic Acid Extraction Protocols to ProGastro SSCS Assay for Detection of Bacterial Enteric Pathogens.

Authors:  Erik Munson; Maureen Napierala; Kimber L Munson; Dorothy Bilbo; Michael A Schulte
Journal:  J Clin Microbiol       Date:  2016-03-02       Impact factor: 5.948

Review 3.  Infectious Disease Management through Point-of-Care Personalized Medicine Molecular Diagnostic Technologies.

Authors:  Luc Bissonnette; Michel G Bergeron
Journal:  J Pers Med       Date:  2012-05-02
  3 in total

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