Literature DB >> 2139227

Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli.

A J Driessen1, W Wickner.   

Abstract

The SecY protein and other membrane proteins of Escherichia coli were solubilized by mixed micelles of n-octyl beta-D-glucopyranoside, phospholipids, and glycerol. Proteoliposomes formed from this extract by detergent dialysis supported energy-dependent translocation and processing of pro-OmpA. Translocation required ATP, SecY, and SecA and was stimulated by a proton-motive force. These results provide an important assay for the isolation and identification of membrane components involved in protein translocation.

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Year:  1990        PMID： 2139227      PMCID： PMC53843          DOI： 10.1073/pnas.87.8.3107

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  43 in total

1.  The ATPase activity of SecA is regulated by acidic phospholipids, SecY, and the leader and mature domains of precursor proteins.

Authors:  R Lill; W Dowhan; W Wickner
Journal:  Cell       Date:  1990-01-26       Impact factor: 41.582

2.  Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein.

Authors:  C N Chang; G Blobel; P Model
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

3.  Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots.

Authors:  G Rouser; S Fkeischer; A Yamamoto
Journal:  Lipids       Date:  1970-05       Impact factor: 1.880

4.  Solubilization of functional membrane proteins.

Authors:  L M Hjelmeland; A Chrambach
Journal:  Methods Enzymol       Date:  1984       Impact factor: 1.600

5.  The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.

Authors:  D P Cerretti; D Dean; G R Davis; D M Bedwell; M Nomura
Journal:  Nucleic Acids Res       Date:  1983-05-11       Impact factor: 16.971

6.  Purification of the precursor form of maltose-binding protein, a periplasmic protein of Escherichia coli.

Authors:  K Ito
Journal:  J Biol Chem       Date:  1982-09-10       Impact factor: 5.157

7.  Solubilization and reconstitution of the lactose transport system from Escherichia coli.

Authors:  M J Newman; T H Wilson
Journal:  J Biol Chem       Date:  1980-11-25       Impact factor: 5.157

8.  Suppressor mutations that restore export of a protein with a defective signal sequence.

Authors:  S D Emr; S Hanley-Way; T J Silhavy
Journal:  Cell       Date:  1981-01       Impact factor: 41.582

9.  Regulation of a membrane component required for protein secretion in Escherichia coli.

Authors:  D B Oliver; J Beckwith
Journal:  Cell       Date:  1982-08       Impact factor: 41.582

10.  A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

Authors:  K Shiba; K Ito; T Yura; D P Cerretti
Journal:  EMBO J       Date:  1984-03       Impact factor: 11.598
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  9 in total

1.  A sporulation membrane protein tethers the pro-sigmaK processing enzyme to its inhibitor and dictates its subcellular localization.

Authors:  David Z Rudner; Richard Losick
Journal:  Genes Dev       Date:  2002-04-15       Impact factor: 11.361

2.  Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.

Authors:  J Akimaru; S Matsuyama; H Tokuda; S Mizushima
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-01       Impact factor: 11.205

3.  Proton transfer is rate-limiting for translocation of precursor proteins by the Escherichia coli translocase.

Authors:  A J Driessen; W Wickner
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

4.  Subunit dynamics in Escherichia coli preprotein translocase.

Authors:  J C Joly; M R Leonard; W T Wickner
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-24       Impact factor: 11.205

5.  Interaction of wild-type signal sequences and their charged variants with model and natural membranes.

Authors:  N M Rao; R Nagaraj
Journal:  Biochem J       Date:  1993-07-01       Impact factor: 3.857

Review 6.  How proteins cross the bacterial cytoplasmic membrane.

Authors:  A J Driessen
Journal:  J Membr Biol       Date:  1994-11       Impact factor: 1.843

Review 7.  The complete general secretory pathway in gram-negative bacteria.

Authors:  A P Pugsley
Journal:  Microbiol Rev       Date:  1993-03

8.  Liposome technology for industrial purposes.

Authors:  Andreas Wagner; Karola Vorauer-Uhl
Journal:  J Drug Deliv       Date:  2010-12-05

9.  Reconstitution of protein translocation from solubilized yeast membranes reveals topologically distinct roles for BiP and cytosolic Hsc70.

Authors:  J L Brodsky; S Hamamoto; D Feldheim; R Schekman
Journal:  J Cell Biol       Date:  1993-01       Impact factor: 10.539
  9 in total

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