Literature DB >> 8197122

Subunit dynamics in Escherichia coli preprotein translocase.

J C Joly1, M R Leonard, W T Wickner.   

Abstract

SecY, SecE, and band 1 copurify as the SecY/E integral membrane domain of Escherichia coli preprotein translocase. To measure the in vivo association of these polypeptides and assay possible exchange, plasmid-borne secY and secE genes were placed under control of the ara regulon and fused to DNA encoding the influenza hemagglutinin epitope. Cells were incubated with [35S]methionine, grown for a "chase" period, and then induced with arabinose to express epitope-tagged, nonradioactive SecY and SecE. Both the wild-type and epitope-tagged polypeptides assembled into functional, heterotrimeric SecY/E complex. However, immunoprecipitation with antibody to the epitope tag did not cross-precipitate radiolabeled SecY or SecE. Thus, these subunits normally associate stably in vivo.

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Year:  1994        PMID： 8197122      PMCID： PMC43856          DOI： 10.1073/pnas.91.11.4703

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  31 in total

1.  PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli.

Authors:  K L Bieker; T J Silhavy
Journal:  Cell       Date:  1990-06-01       Impact factor: 41.582

2.  Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli.

Authors:  A J Driessen; W Wickner
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

3.  Dynamic nature of the quaternary structure of the vesicular stomatitis virus envelope glycoprotein.

Authors:  D S Lyles; V A Varela; J W Parce
Journal:  Biochemistry       Date:  1990-03-13       Impact factor: 3.162

Review 4.  Microtubule dynamics and kinetochore function in mitosis.

Authors:  T J Mitchison
Journal:  Annu Rev Cell Biol       Date:  1988

5.  Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli.

Authors:  P B Wolfe; M Rice; W Wickner
Journal:  J Biol Chem       Date:  1985-02-10       Impact factor: 5.157

6.  Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins.

Authors:  M Watanabe; G Blobel
Journal:  Proc Natl Acad Sci U S A       Date:  1989-03       Impact factor: 11.205

7.  Subunit interactions of class I histocompatibility antigens.

Authors:  K C Parker; J L Strominger
Journal:  Biochemistry       Date:  1985-09-24       Impact factor: 3.162

8.  Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.

Authors:  J Field; J Nikawa; D Broek; B MacDonald; L Rodgers; I A Wilson; R A Lerner; M Wigler
Journal:  Mol Cell Biol       Date:  1988-05       Impact factor: 4.272

9.  SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

Authors:  R Lill; K Cunningham; L A Brundage; K Ito; D Oliver; W Wickner
Journal:  EMBO J       Date:  1989-03       Impact factor: 11.598

10.  A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

Authors:  K Shiba; K Ito; T Yura; D P Cerretti
Journal:  EMBO J       Date:  1984-03       Impact factor: 11.598
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  24 in total

1.  The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits.

Authors:  F Duong; W Wickner
Journal:  EMBO J       Date:  1999-06-15       Impact factor: 11.598

2.  SecYEG assembles into a tetramer to form the active protein translocation channel.

Authors:  E H Manting; C van Der Does; H Remigy; A Engel; A J Driessen
Journal:  EMBO J       Date:  2000-03-01       Impact factor: 11.598

Review 3.  Sec-dependent protein export and the involvement of the molecular chaperone SecB.

Authors:  J Kim; D A Kendall
Journal:  Cell Stress Chaperones       Date:  2000-10       Impact factor: 3.667

4.  Evaluating the oligomeric state of SecYEG in preprotein translocase.

Authors:  T L Yahr; W T Wickner
Journal:  EMBO J       Date:  2000-08-15       Impact factor: 11.598

5.  The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure.

Authors:  Pascal Bessonneau; Véronique Besson; Ian Collinson; Franck Duong
Journal:  EMBO J       Date:  2002-03-01       Impact factor: 11.598

6.  Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking.

Authors:  C R Harris; T J Silhavy
Journal:  J Bacteriol       Date:  1999-06       Impact factor: 3.490

7.  The action of cardiolipin on the bacterial translocon.

Authors:  Vicki A M Gold; Alice Robson; Huan Bao; Tatyana Romantsov; Franck Duong; Ian Collinson
Journal:  Proc Natl Acad Sci U S A       Date:  2010-05-17       Impact factor: 11.205

Review 8.  Oligomeric states of the SecA and SecYEG core components of the bacterial Sec translocon.

Authors:  Sharyn L Rusch; Debra A Kendall
Journal:  Biochim Biophys Acta       Date:  2006-08-30

9.  Expression of the iron-activated nspA and secY genes in Neisseria meningitidis group B by Fur-dependent and -independent mechanisms.

Authors:  Yazdani B Shaik; Susan Grogan; Michael Davey; Shite Sebastian; Sulip Goswami; Borys Szmigielski; Caroline A Genco
Journal:  J Bacteriol       Date:  2006-11-03       Impact factor: 3.490

10.  FtsH is required for proteolytic elimination of uncomplexed forms of SecY, an essential protein translocase subunit.

Authors:  A Kihara; Y Akiyama; K Ito
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-09       Impact factor: 11.205
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