Literature DB >> 2139226

A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein.

M A Garcia-Blanco1, G J Anderson, J Beggs, P A Sharp.   

Abstract

A mammalian protein of approximately 220 kDa (p220) was UV-crosslinked to precursor mRNAs (pre-mRNAs) under splicing conditions. The kinetics and biochemical requirements of the UV-crosslinking of p220 corresponded to the kinetics and biochemical requirements of spliceosome formation. On Western blots, antibodies against the yeast splicing factor PRP8 recognized a doublet of proteins, the faster migrating of which comigrated with p220. Furthermore, UV-crosslinked p220 was immunoprecipitated with anti-PRP8 antisera. These results suggest structural conservation of the splicing factor PRP8 from yeast to mammals and show that this protein is in close proximity to the pre-mRNA in the spliceosome.

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Year:  1990        PMID： 2139226      PMCID： PMC53838          DOI： 10.1073/pnas.87.8.3082

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  35 in total

1.  Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein.

Authors:  D L Black; J A Steitz
Journal:  Cell       Date:  1986-08-29       Impact factor: 41.582

2.  A multicomponent complex is involved in the splicing of messenger RNA precursors.

Authors:  P J Grabowski; S R Seiler; P A Sharp
Journal:  Cell       Date:  1985-08       Impact factor: 41.582

Review 3.  Splicing of messenger RNA precursors.

Authors:  R A Padgett; P J Grabowski; M M Konarska; S Seiler; P A Sharp
Journal:  Annu Rev Biochem       Date:  1986       Impact factor: 23.643

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors:  D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

6.  Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate.

Authors:  P J Grabowski; R A Padgett; P A Sharp
Journal:  Cell       Date:  1984-06       Impact factor: 41.582

7.  Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.

Authors:  M A García-Blanco; S F Jamison; P A Sharp
Journal:  Genes Dev       Date:  1989-12       Impact factor: 11.361

8.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

9.  Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.

Authors:  E A Lerner; M R Lerner; C A Janeway; J A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

10.  Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.

Authors:  A R Krainer; T Maniatis
Journal:  Cell       Date:  1985-10       Impact factor: 41.582
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  25 in total

1.  Antisense oligonucleotide binding to U5 snRNP induces a conformational change that exposes the conserved loop of U5 snRNA.

Authors:  G Ast; A M Weiner
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

2.  Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43.

Authors:  S Gee; S W Krauss; E Miller; K Aoyagi; J Arenas; J G Conboy
Journal:  Proc Natl Acad Sci U S A       Date:  1997-10-28       Impact factor: 11.205

3.  Polypeptide components of Drosophila small nuclear ribonucleoprotein particles.

Authors:  T Paterson; J D Beggs; D J Finnegan; R Lührmann
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971

4.  The canonical GU dinucleotide at the 5' splice site is recognized by p220 of the U5 snRNP within the spliceosome.

Authors:  J L Reyes; P Kois; B B Konforti; M M Konarska
Journal:  RNA       Date:  1996-03       Impact factor: 4.942

5.  Identification of an RNA-dependent ATPase activity in mammalian U5 snRNPs.

Authors:  B Laggerbauer; J Lauber; R Lührmann
Journal:  Nucleic Acids Res       Date:  1996-03-01       Impact factor: 16.971

Review 6.  Yeast protein splicing factors involved in nuclear pre-mRNA splicing.

Authors:  J D Beggs
Journal:  Mol Biol Rep       Date:  1993-08       Impact factor: 2.316

7.  The nuclear matrix phosphoprotein p255 associates with splicing complexes as part of the [U4/U6.U5] tri-snRNP particle.

Authors:  B Chabot; S Bisotto; M Vincent
Journal:  Nucleic Acids Res       Date:  1995-08-25       Impact factor: 16.971

Review 8.  Prp8 protein: at the heart of the spliceosome.

Authors:  Richard J Grainger; Jean D Beggs
Journal:  RNA       Date:  2005-05       Impact factor: 4.942

9.  Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly.

Authors:  D Staknis; R Reed
Journal:  Mol Cell Biol       Date:  1994-05       Impact factor: 4.272

10.  An ATP-independent U2 small nuclear ribonucleoprotein particle/precursor mRNA complex requires both splice sites and the polypyrimidine tract.

Authors:  S F Jamison; M A Garcia-Blanco
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-15       Impact factor: 11.205
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