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Literature DB >> 21378037

Generation and screening of Pichia pastoris strains with enhanced protein production by use of microengraving.

Vasiliki Panagiotou¹, Kerry Routenberg Love, Bo Jiang, Juergen Nett, Terrance Stadheim, J Christopher Love.

Abstract

The selection of highly productive cell lines remains a key step for manufacturing therapeutic proteins. Microengraving was used to screen chemically mutagenized populations of Pichia pastoris for increased production of an Fc fragment. Clones retrieved following three rounds of mutagenesis yielded titers 2.65-fold greater than those of the parental strain.

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Year: 2011 PMID： 21378037 PMCID： PMC3126391 DOI： 10.1128/AEM.00104-11

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

15 in total

Review 1. Process economics of industrial monoclonal antibody manufacture.

Authors: Suzanne S Farid
Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2006-08-08 Impact factor: 3.205

2. A microengraving method for rapid selection of single cells producing antigen-specific antibodies.

Authors: J Christopher Love; Jehnna L Ronan; Gijsbert M Grotenbreg; Annemarthe G van der Veen; Hidde L Ploegh
Journal: Nat Biotechnol Date: 2006-05-14 Impact factor: 54.908

Review 3. Expression of recombinant proteins in Pichia pastoris.

Authors: Pingzuo Li; Anukanth Anumanthan; Xiu-Gong Gao; Kuppusamy Ilangovan; Vincent V Suzara; Nejat Düzgüneş; V Renugopalakrishnan
Journal: Appl Biochem Biotechnol Date: 2007-08 Impact factor: 2.926

Review 4. Selection methods for high-producing mammalian cell lines.

Authors: Susan M Browne; Mohamed Al-Rubeai
Journal: Trends Biotechnol Date: 2007-07-30 Impact factor: 19.536

Review 5. Advances in protein therapeutics.

Authors: Gary Woodnutt; Bernard Violand; Michael North
Journal: Curr Opin Drug Discov Devel Date: 2008-11

Review 6. Mutagenesis in yeast.

Authors: J F Spencer; D M Spencer
Journal: Methods Mol Biol Date: 1996

Review 7. Heterologous protein expression in the methylotrophic yeast Pichia pastoris.

Authors: J L Cereghino; J M Cregg
Journal: FEMS Microbiol Rev Date: 2000-01 Impact factor: 16.408

8. Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.

Authors: Kerry Routenberg Love; Vasiliki Panagiotou; Bo Jiang; Terrance A Stadheim; J Christopher Love
Journal: Biotechnol Bioeng Date: 2010-06-01 Impact factor: 4.530

9. Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving.

Authors: Qing Han; Elizabeth M Bradshaw; Björn Nilsson; David A Hafler; J Christopher Love
Journal: Lab Chip Date: 2010-04-08 Impact factor: 6.799

Review 10. In pursuit of a super producer-alternative paths to high producing recombinant mammalian cells.

Authors: Gargi Seth; Salim Charaniya; Katie F Wlaschin; Wei-Shou Hu
Journal: Curr Opin Biotechnol Date: 2007-12-21 Impact factor: 9.740

8 in total

1. Single cells from human primary colorectal tumors exhibit polyfunctional heterogeneity in secretions of ELR+ CXC chemokines.

Authors: Viktor A Adalsteinsson; Narmin Tahirova; Naren Tallapragada; Xiaosai Yao; Liam Campion; Alessandro Angelini; Thomas B Douce; Cindy Huang; Brittany Bowman; Christina A Williamson; Douglas S Kwon; K Dane Wittrup; J Christopher Love
Journal: Integr Biol (Camb) Date: 2013-10 Impact factor: 2.192

Generation and screening of Pichia pastoris strains with enhanced protein production by use of microengraving.

Review 1. Process economics of industrial monoclonal antibody manufacture.

2. A microengraving method for rapid selection of single cells producing antigen-specific antibodies.

Review 3. Expression of recombinant proteins in Pichia pastoris.

Review 4. Selection methods for high-producing mammalian cell lines.

Review 5. Advances in protein therapeutics.

Review 6. Mutagenesis in yeast.

Review 7. Heterologous protein expression in the methylotrophic yeast Pichia pastoris.

8. Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.

9. Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving.

Review 10. In pursuit of a super producer-alternative paths to high producing recombinant mammalian cells.

1. Single cells from human primary colorectal tumors exhibit polyfunctional heterogeneity in secretions of ELR+ CXC chemokines.

2. Stochastic particle barcoding for single-cell tracking and multiparametric analysis.

3. Crossword: a fully automated algorithm for the segmentation and quality control of protein microarray images.

4. Nanowell-based immunoassays for measuring single-cell secretion: characterization of transport and surface binding.

Review 5. Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization.

6. Going native: Direct high throughput screening of secreted full-length IgG antibodies against cell membrane proteins.

7. Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris.

8. A Pichia biosensor for high-throughput analyses of compounds that can influence mosquito behavior.