| Literature DB >> 18025573 |
Pingzuo Li1, Anukanth Anumanthan, Xiu-Gong Gao, Kuppusamy Ilangovan, Vincent V Suzara, Nejat Düzgüneş, V Renugopalakrishnan.
Abstract
Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode.Entities:
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Year: 2007 PMID： 18025573 DOI： 10.1007/s12010-007-0003-x
Source DB: PubMed Journal: Appl Biochem Biotechnol ISSN： 0273-2289 Impact factor: 2.926