Literature DB >> 2136831

Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes.

A Bindereif1, T Wolff, M R Green.   

Abstract

U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.

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Year:  1990        PMID： 2136831      PMCID： PMC551655          DOI： 10.1002/j.1460-2075.1990.tb08102.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  26 in total

1.  Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes.

Authors:  M M Konarska; P A Sharp
Journal:  Cell       Date:  1987-06-19       Impact factor: 41.582

2.  Direct chemical method for sequencing RNA.

Authors:  D A Peattie
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

3.  Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.

Authors:  C W Pikielny; B C Rymond; M Rosbash
Journal:  Nature       Date:  1986 Nov 27-Dec 3       Impact factor: 49.962

4.  Specific labeling of 3' termini of RNA with T4 RNA ligase.

Authors:  T E England; A G Bruce; O C Uhlenbeck
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

5.  A chemical modification/interference study of yeast pre-mRNA spliceosome assembly and splicing.

Authors:  B C Rymond; M Rosbash
Journal:  Genes Dev       Date:  1988-04       Impact factor: 11.361

6.  Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles.

Authors:  M Zillmann; M L Zapp; S M Berget
Journal:  Mol Cell Biol       Date:  1988-02       Impact factor: 4.272

7.  Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.

Authors:  A I Lamond; M M Konarska; P J Grabowski; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1988-01       Impact factor: 11.205

8.  Spliceosome assembly in yeast.

Authors:  S C Cheng; J Abelson
Journal:  Genes Dev       Date:  1987-11       Impact factor: 11.361

9.  An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.

Authors:  A Bindereif; M R Green
Journal:  EMBO J       Date:  1987-08       Impact factor: 11.598

10.  Analysis of mRNA 3' end formation by modification interference: the only modifications which prevent processing lie in AAUAAA and the poly(A) site.

Authors:  L Conway; M Wickens
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598
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  19 in total

1.  Domain structure of U2 and U4/U6 small nuclear ribonucleoprotein particles from Trypanosoma brucei: identification of trans-spliceosomal specific RNA-protein interactions.

Authors:  A Günzl; M Cross; A Bindereif
Journal:  Mol Cell Biol       Date:  1992-02       Impact factor: 4.272

2.  Thiophosphates in yeast U6 snRNA specifically affect pre-mRNA splicing in vitro.

Authors:  P Fabrizio; J Abelson
Journal:  Nucleic Acids Res       Date:  1992-07-25       Impact factor: 16.971

3.  A new U6 small nuclear ribonucleoprotein-specific protein conserved between cis- and trans-splicing systems.

Authors:  K Gröning; Z Palfi; S Gupta; M Cross; T Wolff; A Bindereif
Journal:  Mol Cell Biol       Date:  1991-04       Impact factor: 4.272

4.  Recombinant Epstein-Barr virus with small RNA (EBER) genes deleted transforms lymphocytes and replicates in vitro.

Authors:  S Swaminathan; B Tomkinson; E Kieff
Journal:  Proc Natl Acad Sci U S A       Date:  1991-02-15       Impact factor: 11.205

5.  Conserved domains of human U4 snRNA required for snRNP and spliceosome assembly.

Authors:  C Wersig; A Bindereif
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

6.  A discrete 3' region of U6 small nuclear RNA modulates the phosphorylation cycle of the C1 heterogeneous nuclear ribonucleoprotein particle protein.

Authors:  S H Mayrand; P A Fung; T Pederson
Journal:  Mol Cell Biol       Date:  1996-03       Impact factor: 4.272

7.  p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor.

Authors:  Mathias Bell; Silke Schreiner; Andrey Damianov; Ram Reddy; Albrecht Bindereif
Journal:  EMBO J       Date:  2002-06-03       Impact factor: 11.598

8.  Human U4/U6 snRNP recycling factor p110: mutational analysis reveals the function of the tetratricopeptide repeat domain in recycling.

Authors:  Jan Medenbach; Silke Schreiner; Sunbin Liu; Reinhard Lührmann; Albrecht Bindereif
Journal:  Mol Cell Biol       Date:  2004-09       Impact factor: 4.272

9.  The low-abundance U11 and U12 small nuclear ribonucleoproteins (snRNPs) interact to form a two-snRNP complex.

Authors:  K M Wassarman; J A Steitz
Journal:  Mol Cell Biol       Date:  1992-03       Impact factor: 4.272

10.  Recycling of the U12-type spliceosome requires p110, a component of the U6atac snRNP.

Authors:  Andrey Damianov; Silke Schreiner; Albrecht Bindereif
Journal:  Mol Cell Biol       Date:  2004-02       Impact factor: 4.272
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