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Literature DB >> 3537805

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.

Abstract

Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.

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Year: 1986 PMID： 3537805 DOI： 10.1038/324341a0

Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962

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Cited

128 in total

1. Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels.

Authors: R Das; R Reed
Journal: RNA Date: 1999-11 Impact factor: 4.942

2. Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing.

Authors: H Du; M Rosbash
Journal: RNA Date: 2001-01 Impact factor: 4.942

3. Functional and physical interactions between components of the Prp19p-associated complex.

Authors: Chun-Hong Chen; Wan-Chin Yu; Twee Y Tsao; Lian-Yung Wang; Hau-Ren Chen; Jui-Yen Lin; Wei-Yü Tsai; Soo-Chen Cheng
Journal: Nucleic Acids Res Date: 2002-02-15 Impact factor: 16.971

4. cis-acting sequences involved in exon selection in the chicken beta-tropomyosin gene.

Authors: M E Gallego; L Balvay; E Brody
Journal: Mol Cell Biol Date: 1992-12 Impact factor: 4.272

5. Evidence for a base-pairing interaction between U6 small nuclear RNA and 5' splice site during the splicing reaction in yeast.

Authors: H Sawa; J Abelson
Journal: Proc Natl Acad Sci U S A Date: 1992-12-01 Impact factor: 11.205

6. The 5' splice site consensus RNA oligonucleotide induces assembly of U2/U4/U5/U6 small nuclear ribonucleoprotein complexes.

Authors: K B Hall; M M Konarska
Journal: Proc Natl Acad Sci U S A Date: 1992-11-15 Impact factor: 11.205

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.

1. Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels.

2. Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing.

3. Functional and physical interactions between components of the Prp19p-associated complex.

4. cis-acting sequences involved in exon selection in the chicken beta-tropomyosin gene.

5. Evidence for a base-pairing interaction between U6 small nuclear RNA and 5' splice site during the splicing reaction in yeast.

6. The 5' splice site consensus RNA oligonucleotide induces assembly of U2/U4/U5/U6 small nuclear ribonucleoprotein complexes.

7. The spliceosome assembly pathway in mammalian extracts.

8. Requirements for U2 snRNP addition to yeast pre-mRNA.

9. UACUAAC is the preferred branch site for mammalian mRNA splicing.

10. Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus.