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Literature DB >> 21331889

Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA.

Zhe Ren¹, Shen Li, Qiao-li Wang, Yang-fei Xiang, Yun-xia Cui, Yi-fei Wang, Ren-bin Qi, Da-xiang Lu, Shu-min Zhang, Pei-zhuo Zhang.

Abstract

RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40, respectively. In this study, we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.

Entities: Gene Species

Mesh：

Substances：

Year: 2011 PMID： 21331889 PMCID： PMC8222430 DOI： 10.1007/s12250-011-3162-9

Source DB: PubMed Journal: Virol Sin ISSN： 1995-820X Impact factor: 4.327

32 in total

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6. Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference.

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7. Analysis of gene function in somatic mammalian cells using small interfering RNAs.

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8. siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells.

Authors: Hiroaki Kawasaki; Eigo Suyama; Mayu Iyo; Kazunari Taira
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Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA.

1. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

2. Identification and characterization of a herpes simplex virus gene product required for encapsidation of virus DNA.

Review 3. The enzyme ribonucleotide reductase: target for antitumor and anti-HIV therapy.

4. Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor.

5. RNA interference targeting Fas protects mice from fulminant hepatitis.

6. Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference.

7. Analysis of gene function in somatic mammalian cells using small interfering RNAs.

8. siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells.

9. Deletion of the herpes simplex virus type 1 ribonucleotide reductase gene alters virulence and latency in vivo.

10. Effect of siRNA on HSV-1 plaque formation and relative expression levels of UL39 mRNA.

1. Developing oncolytic Herpes simplex virus type 1 through UL39 knockout by CRISPR-Cas9.

2. Adenovirus-mediated shRNA interference against HSV-1 replication in vitro.

3. RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions.