Literature DB >> 18551244

Effect of siRNA on HSV-1 plaque formation and relative expression levels of UL39 mRNA.

Ren Zhe1, Zhang Mei-Ying, Kaio Kitazato, Nobuyuki Kobayash, Zhu Qin-Chang, Zhang Pei-Zhuo, Yang Zhi-Rong, Wang Yi-Fei.   

Abstract

RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. In this study, we applied siRNAs targeting the UL39 gene of human herpes simplex virus type 1 (HSV-1), which encodes the large subunit of ribonucleotide reductase, ICP6. Using an ICP6 expression reporter plasmid and an in vitro model of infection, we showed that synthetic siRNA silenced effectively and specifically UL39 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.

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Year:  2008        PMID： 18551244     DOI： 10.1007/s00705-008-0110-1

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  9 in total

1.  Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA.

Authors:  Zhe Ren; Shen Li; Qiao-li Wang; Yang-fei Xiang; Yun-xia Cui; Yi-fei Wang; Ren-bin Qi; Da-xiang Lu; Shu-min Zhang; Pei-zhuo Zhang
Journal:  Virol Sin       Date:  2011-02-18       Impact factor: 4.327

2.  Adenovirus-mediated shRNA interference against HSV-1 replication in vitro.

Authors:  Bo Song; Xinjing Liu; Qingzhi Wang; Rui Zhang; Ting Yang; Zhiqiang Han; Yuming Xu
Journal:  J Neurovirol       Date:  2016-08-26       Impact factor: 2.643

3.  An important role for syndecan-1 in herpes simplex virus type-1 induced cell-to-cell fusion and virus spread.

Authors:  Ghadah A Karasneh; Mohamed Ali; Deepak Shukla
Journal:  PLoS One       Date:  2011-09-21       Impact factor: 3.240

4.  Silencing herpes simplex virus type 1 capsid protein encoding genes by siRNA: a promising antiviral therapeutic approach.

Authors:  Fujun Jin; Shen Li; Kai Zheng; Cuiqin Zhuo; Kaiqi Ma; Maoyun Chen; Qiaoli Wang; Peizhuo Zhang; Jianglin Fan; Zhe Ren; Yifei Wang
Journal:  PLoS One       Date:  2014-05-02       Impact factor: 3.240

5.  Small interfering RNA targeting for infected-cell polypeptide 4 inhibits herpes simplex virus type 1 replication in retinal pigment epithelial cells.

Authors:  Fang Duan; Shiming Ni; Yuhong Nie; Qiang Huang; Kaili Wu
Journal:  Clin Exp Ophthalmol       Date:  2011-10-20       Impact factor: 4.207

6.  Analysis of virion-incorporated host proteins required for herpes simplex virus type 1 infection through a RNA interference screen.

Authors:  Camille Stegen; Yordanka Yakova; Daniel Henaff; Julien Nadjar; Johanne Duron; Roger Lippé
Journal:  PLoS One       Date:  2013-01-03       Impact factor: 3.240

7.  Targeting herpetic keratitis by gene therapy.

Authors:  Hossein Mostafa Elbadawy; Marine Gailledrat; Carole Desseaux; Diego Ponzin; Stefano Ferrari
Journal:  J Ophthalmol       Date:  2012-12-26       Impact factor: 1.909

8.  Tin oxide nanowires suppress herpes simplex virus-1 entry and cell-to-cell membrane fusion.

Authors:  James Trigilio; Thessicar E Antoine; Ingo Paulowicz; Yogendra K Mishra; Rainer Adelung; Deepak Shukla
Journal:  PLoS One       Date:  2012-10-24       Impact factor: 3.240

9.  RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions.

Authors:  Amanda Perse da Silva; Juliana Freitas Lopes; Vanessa Salete de Paula
Journal:  Braz J Infect Dis       Date:  2014-05-15       Impact factor: 3.257
  9 in total

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