Literature DB >> 21248091

Nucleic Acid Amplification of the opa Gene for Detection of Neisseria gonorrhoeae: experience from a diagnostic laboratory.

M J Maze1, Sheryl Young, Julie Creighton, Trevor Anderson, Anja Werno.   

Abstract

We report results of Neisseria gonorrhoeae nucleic acid amplification testing (NAAT) with the Abbott m2000 PCR at a tertiary laboratory 6 months after its introduction. Of 5,475 specimens tested, 45 samples (0.82%) tested positive for N. gonorrhoeae. Eight were not cultured, but seven tested positive with a porA pseudogene NAAT.

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Year:  2011        PMID： 21248091      PMCID： PMC3067690          DOI： 10.1128/JCM.01624-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  Confirmation by 16S rRNA PCR of the COBAS AMPLICOR CT/NG test for diagnosis of Neisseria gonorrhoeae infection in a low-prevalence population.

Authors:  David J Diemert; Michael D Libman; Pierre Lebel
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

2.  False-positive gonorrhea test results with a nucleic acid amplification test: the impact of low prevalence on positive predictive value.

Authors:  Alan R Katz; Paul V Effler; Roy G Ohye; Barbara Brouillet; Maria Veneranda C Lee; Peter M Whiticar
Journal:  Clin Infect Dis       Date:  2004-02-27       Impact factor: 9.079

3.  The NAAT is out of the bag.

Authors:  Jeffrey D Klausner
Journal:  Clin Infect Dis       Date:  2004-02-27       Impact factor: 9.079

4.  Sexually transmitted infections in New Zealand in 2003.

Authors:  Alisha Johnston; Dinusha Fernando; Graham MacBride-Stewart
Journal:  N Z Med J       Date:  2005-03-11

5.  Characteristics of the m2000 automated sample preparation and multiplex real-time PCR system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae.

Authors:  R Marshall; M Chernesky; D Jang; E W Hook; C P Cartwright; B Howell-Adams; S Ho; J Welk; J Lai-Zhang; J Brashear; B Diedrich; K Otis; E Webb; J Robinson; H Yu
Journal:  J Clin Microbiol       Date:  2007-01-03       Impact factor: 5.948

6.  Diagnostic value of molecular confirmation assays for Neisseria gonorrhoeae.

Authors:  Silke Peter-Getzlaff; Jacqueline Luethy; Burkhard Springer
Journal:  J Clin Microbiol       Date:  2007-11       Impact factor: 5.948

7.  Evaluation of three automated nucleic acid amplification systems for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in first-void urine specimens.

Authors:  P N Levett; K Brandt; K Olenius; C Brown; K Montgomery; G B Horsman
Journal:  J Clin Microbiol       Date:  2008-04-09       Impact factor: 5.948

8.  A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene.

Authors:  D M Whiley; P J Buda; J Bayliss; L Cover; J Bates; T P Sloots
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-07-10       Impact factor: 3.267

9.  LCR testing for gonorrhoea and chlamydia in population surveys and other screenings of low prevalence populations: coping with decreased positive predictive value.

Authors:  J M Zenilman; W C Miller; C Gaydos; S M Rogers; C F Turner
Journal:  Sex Transm Infect       Date:  2003-04       Impact factor: 3.519

10.  Neisseria species identification assay for the confirmation of Neisseria gonorrhoeae-positive results of the COBAS Amplicor PCR.

Authors:  Kathy A Mangold; MaryAnn Regner; Mohammed Tajuddin; Aamair M Tajuddin; Lawrence Jennings; Hongyan Du; Karen L Kaul
Journal:  J Clin Microbiol       Date:  2007-03-14       Impact factor: 5.948
  10 in total
  3 in total

1.  Evaluation of six commercial nucleic acid amplification tests for detection of Neisseria gonorrhoeae and other Neisseria species.

Authors:  Sepehr N Tabrizi; Magnus Unemo; Athena E Limnios; Tiffany R Hogan; Stig-Ove Hjelmevoll; Susanne M Garland; John Tapsall
Journal:  J Clin Microbiol       Date:  2011-08-03       Impact factor: 5.948

2.  A portable magnetofluidic platform for detecting sexually transmitted infections and antimicrobial susceptibility.

Authors:  Alexander Y Trick; Johan H Melendez; Fan-En Chen; Liben Chen; Annet Onzia; Aidah Zawedde; Edith Nakku-Joloba; Peter Kyambadde; Emmanuel Mande; Joshua Matovu; Maxine Atuheirwe; Richard Kwizera; Elizabeth A Gilliams; Yu-Hsiang Hsieh; Charlotte A Gaydos; Yukari C Manabe; Matthew M Hamill; Tza-Huei Wang
Journal:  Sci Transl Med       Date:  2021-05-12       Impact factor: 17.956

3.  Loop-mediated isothermal amplification of Neisseria gonorrhoeae porA pseudogene: a rapid and reliable method to detect gonorrhea.

Authors:  Mei-Ling Liu; Yong Xia; Xing-Zhong Wu; Jian-Qiong Huang; Xu-Guang Guo
Journal:  AMB Express       Date:  2017-02-23       Impact factor: 3.298
  3 in total

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