OBJECTIVE: We investigated associations of early pregnancy maternal vitamin D concentrations with differential gene expression and post-transcription regulation. METHOD: Plasma 25-hydroxyvitamin D (25[OH]D) was measured among participants of a nested case-control study. Participants with low (<25.5 ng/ml) and high (≥31.7 ng/ml) 25[OH]D were identified among controls. Peripheral blood messenger RNA (mRNA) (N = 21) and microRNA (miRNA) (N = 13) expression studies were conducted among participants with low and high 25[OH]D concentrations. Differential expression between low/high groups were evaluated using Student's t-test, fold change, and SAM comparisons. We further investigated functions and functional relationships of differentially expressed mRNAs and targets of differentially expressed miRNAs. RESULTS: Three hundred and five genes (299 upregulated and 6 downregulated) and 11 miRNAs (10 downregulated and 1 upregulated) were differentially expressed among participants with low 25[OH]D compared with those who had high 25[OH]D. Genes that participate in a wide range of cellular functions, including organ and system development (e.g. angiogenesis), inflammation and metabolic processes (e.g. carbohydrate/lipid metabolism), as well as miRNAs that target these genes were differentially expressed among women with low 25[OH]D compared with those with high 25[OH]D. CONCLUSION: Early pregnancy plasma 25[OH]D concentrations are associated with maternal peripheral blood gene expression and post-transcription regulation.
OBJECTIVE: We investigated associations of early pregnancy maternal vitamin D concentrations with differential gene expression and post-transcription regulation. METHOD: Plasma 25-hydroxyvitamin D (25[OH]D) was measured among participants of a nested case-control study. Participants with low (<25.5 ng/ml) and high (≥31.7 ng/ml) 25[OH]D were identified among controls. Peripheral blood messenger RNA (mRNA) (N = 21) and microRNA (miRNA) (N = 13) expression studies were conducted among participants with low and high 25[OH]D concentrations. Differential expression between low/high groups were evaluated using Student's t-test, fold change, and SAM comparisons. We further investigated functions and functional relationships of differentially expressed mRNAs and targets of differentially expressed miRNAs. RESULTS: Three hundred and five genes (299 upregulated and 6 downregulated) and 11 miRNAs (10 downregulated and 1 upregulated) were differentially expressed among participants with low 25[OH]D compared with those who had high 25[OH]D. Genes that participate in a wide range of cellular functions, including organ and system development (e.g. angiogenesis), inflammation and metabolic processes (e.g. carbohydrate/lipid metabolism), as well as miRNAs that target these genes were differentially expressed among women with low 25[OH]D compared with those with high 25[OH]D. CONCLUSION: Early pregnancy plasma 25[OH]D concentrations are associated with maternal peripheral blood gene expression and post-transcription regulation.
Authors: Hooman Mirzakhani; Augusto A Litonjua; Thomas F McElrath; George O'Connor; Aviva Lee-Parritz; Ronald Iverson; George Macones; Robert C Strunk; Leonard B Bacharier; Robert Zeiger; Bruce W Hollis; Diane E Handy; Amitabh Sharma; Nancy Laranjo; Vincent Carey; Weilliang Qiu; Marc Santolini; Shikang Liu; Divya Chhabra; Daniel A Enquobahrie; Michelle A Williams; Joseph Loscalzo; Scott T Weiss Journal: J Clin Invest Date: 2016-11-14 Impact factor: 14.808
Authors: Jonathan Y Huang; Chunfang Qiu; Raymond S Miller; David S Siscovick; Michelle A Williams; Daniel A Enquobahrie Journal: Paediatr Perinat Epidemiol Date: 2013-07-12 Impact factor: 3.980