Literature DB >> 21212884

Controlled enzymatic synthesis of natural-linkage, defined-length polyubiquitin chains using lysines with removable protecting groups.

Carlos A Castañeda1, Jia Liu, Tanuja R Kashyap, Rajesh K Singh, David Fushman, T Ashton Cropp.   

Abstract

E2 enzymes catalyze the ATP-dependent polymerization of polyubiquitin chains which function as molecular signals in the regulation of numerous cellular processes. Here we present a method that uses genetically encoded unnatural amino acids to halt and re-start ubiquitin polymerization providing access to natural-linkage, precision-length ubiquitin chains that can be used for biochemical, structural, and dynamics studies.

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Year:  2011        PMID： 21212884      PMCID： PMC3190230          DOI： 10.1039/c0cc04868b

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  22 in total

1.  Structural properties of polyubiquitin chains in solution.

Authors:  Ranjani Varadan; Olivier Walker; Cecile Pickart; David Fushman
Journal:  J Mol Biol       Date:  2002-12-06       Impact factor: 5.469

2.  Total chemical synthesis of di-ubiquitin chains.

Authors:  K S Ajish Kumar; Liat Spasser; Lesly A Erlich; Sudhir N Bavikar; Ashraf Brik
Journal:  Angew Chem Int Ed Engl       Date:  2010-11-22       Impact factor: 15.336

Review 3.  Polyubiquitin chains: polymeric protein signals.

Authors:  Cecile M Pickart; David Fushman
Journal:  Curr Opin Chem Biol       Date:  2004-12       Impact factor: 8.822

4.  Controlled synthesis of polyubiquitin chains.

Authors:  Cecile M Pickart; Shahri Raasi
Journal:  Methods Enzymol       Date:  2005       Impact factor: 1.600

5.  Inhibition of the 26 S proteasome by polyubiquitin chains synthesized to have defined lengths.

Authors:  J Piotrowski; R Beal; L Hoffman; K D Wilkinson; R E Cohen; C M Pickart
Journal:  J Biol Chem       Date:  1997-09-19       Impact factor: 5.157

6.  Analysis of interdomain dynamics in a two-domain protein using residual dipolar couplings together with 15N relaxation data.

Authors:  Yaroslav Ryabov; David Fushman
Journal:  Magn Reson Chem       Date:  2006-07       Impact factor: 2.447

7.  In vitro assembly and recognition of Lys-63 polyubiquitin chains.

Authors:  R M Hofmann; C M Pickart
Journal:  J Biol Chem       Date:  2001-05-21       Impact factor: 5.157

8.  Interdomain mobility in di-ubiquitin revealed by NMR.

Authors:  Yaroslav Ryabov; David Fushman
Journal:  Proteins       Date:  2006-06-01

9.  Specificity of the ubiquitin isopeptidase in the PA700 regulatory complex of 26 S proteasomes.

Authors:  Y A Lam; G N DeMartino; C M Pickart; R E Cohen
Journal:  J Biol Chem       Date:  1997-11-07       Impact factor: 5.157

10.  Structure of a diubiquitin conjugate and a model for interaction with ubiquitin conjugating enzyme (E2).

Authors:  W J Cook; L C Jeffrey; M Carson; Z Chen; C M Pickart
Journal:  J Biol Chem       Date:  1992-08-15       Impact factor: 5.157
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  19 in total

1.  Segmental isotopic labeling of ubiquitin chains to unravel monomer-specific molecular behavior.

Authors:  Carlos A Castañeda; Liat Spasser; Sudhir N Bavikar; Ashraf Brik; David Fushman
Journal:  Angew Chem Int Ed Engl       Date:  2011-09-28       Impact factor: 15.336

2.  Genetic encoding of the post-translational modification 2-hydroxyisobutyryl-lysine.

Authors:  William A Knight; T Ashton Cropp
Journal:  Org Biomol Chem       Date:  2015-05-22       Impact factor: 3.876

3.  Analysis of the topology of ubiquitin chains.

Authors:  Lucia Geis-Asteggiante; Amanda E Lee; Catherine Fenselau
Journal:  Methods Enzymol       Date:  2019-07-13       Impact factor: 1.600

4.  Modifying the vicinity of the isopeptide bond to reveal differential behavior of ubiquitin chains with interacting proteins: organic chemistry applied to synthetic proteins.

Authors:  Najat Haj-Yahya; Mahmood Haj-Yahya; Carlos A Castañeda; Liat Spasser; Hosahalli P Hemantha; Muhammad Jbara; Marlin Penner; Aaron Ciechanover; David Fushman; Ashraf Brik
Journal:  Angew Chem Int Ed Engl       Date:  2013-09-04       Impact factor: 15.336

5.  Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins.

Authors:  Carlos A Castañeda; Emma K Dixon; Olivier Walker; Apurva Chaturvedi; Mark A Nakasone; Joseph E Curtis; Megan R Reed; Susan Krueger; T Ashton Cropp; David Fushman
Journal:  Structure       Date:  2016-02-11       Impact factor: 5.006

6.  Nonenzymatic assembly of natural polyubiquitin chains of any linkage composition and isotopic labeling scheme.

Authors:  Carlos Castañeda; Jia Liu; Apurva Chaturvedi; Urszula Nowicka; T Ashton Cropp; David Fushman
Journal:  J Am Chem Soc       Date:  2011-10-19       Impact factor: 15.419

Review 7.  Unraveling the complexity of ubiquitin signaling.

Authors:  Eric R Strieter; David A Korasick
Journal:  ACS Chem Biol       Date:  2012-01-11       Impact factor: 5.100

8.  Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria.

Authors:  Tal Keren-Kaplan; Ilan Attali; Khatereh Motamedchaboki; Brian A Davis; Neta Tanner; Yael Reshef; Einat Laudon; Mikhail Kolot; Olga Levin-Kravets; Oded Kleifeld; Michael Glickman; Bruce F Horazdovsky; Dieter A Wolf; Gali Prag
Journal:  EMBO J       Date:  2011-11-11       Impact factor: 11.598

9.  Alanine scan of core positions in ubiquitin reveals links between dynamics, stability, and function.

Authors:  Shirley Y Lee; Lester Pullen; Daniel J Virgil; Carlos A Castañeda; Dulith Abeykoon; Daniel N A Bolon; David Fushman
Journal:  J Mol Biol       Date:  2013-12-19       Impact factor: 5.469

10.  A Rapid and Versatile Method for Generating Proteins with Defined Ubiquitin Chains.

Authors:  Kirby Martinez-Fonts; Andreas Matouschek
Journal:  Biochemistry       Date:  2016-03-17       Impact factor: 3.162
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