Literature DB >> 2117878

Cloning of the citrate permease gene of Lactococcus lactis subsp. lactis biovar diacetylactis and expression in Escherichia coli.

F Sesma1, D Gardiol, A P de Ruiz Holgado, D de Mendoza.   

Abstract

The citrate plasmid (Cit+ plasmid) from Lactococcus lactis subsp. lactis biovar diacetylactis was cloned into the EcoRI site of plasmid pUC18. This recombinant plasmid enabled Escherichia coli K-12 to transport and utilize citrate as a source of energy, indicating expression of the citrate permease from L. lactis biovar diacetylactis. The citrate permease was under the control of the lac promoter of pUC18. Genetic expression of the Cit+ plasmid in maxicells revealed that the plasmid encoded two polypeptides of 47 and 32 kilodaltons, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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Year:  1990        PMID： 2117878      PMCID： PMC184566          DOI： 10.1128/aem.56.7.2099-2103.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  19 in total

1.  Improved Medium for Detection of Citrate-Fermenting Streptococcus lactis subsp. diacetylactis.

Authors:  G M Kempler; L L McKay
Journal:  Appl Environ Microbiol       Date:  1980-04       Impact factor: 4.792

2.  Oxidation of citrate by Escherichia coli.

Authors:  F J S LARA; J L STOKES
Journal:  J Bacteriol       Date:  1952-03       Impact factor: 3.490

3.  Transfer of Plasmids between Bacillus subtilis and Streptococcus lactis.

Authors:  M Baigorí; F Sesma; A P de Ruiz Holgado; D de Mendoza
Journal:  Appl Environ Microbiol       Date:  1988-05       Impact factor: 4.792

4.  Characterization of Plasmid Deoxyribonucleic Acid in Streptococcus lactis subsp. diacetylactis: Evidence for Plasmid-Linked Citrate Utilization.

Authors:  G M Kempler; L L McKay
Journal:  Appl Environ Microbiol       Date:  1979-02       Impact factor: 4.792

5.  Simple method for identification of plasmid-coded proteins.

Authors:  A Sancar; A M Hack; W D Rupp
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Thermal regulation of membrane fluidity in Escherichia coli. Effects of overproduction of beta-ketoacyl-acyl carrier protein synthase I.

Authors:  D de Mendoza; A Klages Ulrich; J E Cronan
Journal:  J Biol Chem       Date:  1983-02-25       Impact factor: 5.157

8.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

9.  Simple and rapid method for isolating large plasmid DNA from lactic streptococci.

Authors:  D G Anderson; L L McKay
Journal:  Appl Environ Microbiol       Date:  1983-09       Impact factor: 4.792

10.  Expression of cloned genes by in vivo insertion of tac promoter using a mini-Mu bacteriophage.

Authors:  H C Gramajo; A M Viale; D de Mendoza
Journal:  Gene       Date:  1988-05-30       Impact factor: 3.688
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  18 in total

Review 1.  The 2-hydroxycarboxylate transporter family: physiology, structure, and mechanism.

Authors:  Iwona Sobczak; Juke S Lolkema
Journal:  Microbiol Mol Biol Rev       Date:  2005-12       Impact factor: 11.056

Review 2.  Physiology of pyruvate metabolism in Lactococcus lactis.

Authors:  M Cocaign-Bousquet; C Garrigues; P Loubiere; N D Lindley
Journal:  Antonie Van Leeuwenhoek       Date:  1996-10       Impact factor: 2.271

3.  Metabolic and transcriptomic adaptation of Lactococcus lactis subsp. lactis Biovar diacetylactis in response to autoacidification and temperature downshift in skim milk.

Authors:  Sandy Raynaud; Rémi Perrin; Muriel Cocaign-Bousquet; Pascal Loubiere
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

4.  Plasmids of raw milk cheese isolate Lactococcus lactis subsp. lactis biovar diacetylactis DPC3901 suggest a plant-based origin for the strain.

Authors:  Vincenzo Fallico; Olivia McAuliffe; Gerald F Fitzgerald; R Paul Ross
Journal:  Appl Environ Microbiol       Date:  2011-07-29       Impact factor: 4.792

5.  Control of the shift from homolactic acid to mixed-acid fermentation in Lactococcus lactis: predominant role of the NADH/NAD+ ratio.

Authors:  C Garrigues; P Loubiere; N D Lindley; M Cocaign-Bousquet
Journal:  J Bacteriol       Date:  1997-09       Impact factor: 3.490

6.  Processing of as-48ABC RNA in AS-48 enterocin production by Enterococcus faecalis.

Authors:  Matilde Fernández; Marina Sánchez-Hidalgo; Nieves García-Quintáns; Manuel Martínez-Bueno; Eva Valdivia; Paloma López; Mercedes Maqueda
Journal:  J Bacteriol       Date:  2007-11-02       Impact factor: 3.490

7.  Contribution of citrate metabolism to the growth of Lactococcus lactis CRL264 at low pH.

Authors:  Claudia Sánchez; Ana Rute Neves; João Cavalheiro; Margarida Moreira dos Santos; Nieves García-Quintáns; Paloma López; Helena Santos
Journal:  Appl Environ Microbiol       Date:  2007-12-21       Impact factor: 4.792

8.  Acid-inducible transcription of the operon encoding the citrate lyase complex of Lactococcus lactis Biovar diacetylactis CRL264.

Authors:  Mauricio G Martín; Pablo D Sender; Salvador Peirú; Diego de Mendoza; Christian Magni
Journal:  J Bacteriol       Date:  2004-09       Impact factor: 3.490

9.  Uptake of α-ketoglutarate by citrate transporter CitP drives transamination in Lactococcus lactis.

Authors:  Agata M Pudlik; Juke S Lolkema
Journal:  Appl Environ Microbiol       Date:  2012-11-30       Impact factor: 4.792

10.  Transcriptional activation of the citrate permease P gene of Lactococcus lactis biovar diacetylactis by an insertion sequence-like element present in plasmid pCIT264.

Authors:  F López de Felipe; C Magni; D de Mendoza; P López
Journal:  Mol Gen Genet       Date:  1996-03-07
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