Literature DB >> 21175615

Cryo-electron tomography: methodology, developments and biological applications.

D Vanhecke1, S Asano, Z Kochovski, R Fernandez-Busnadiego, N Schrod, W Baumeister, V Lučić.   

Abstract

Cryo-electron tomography allows three-dimensional visualization of frozen-hydrated, vitrified biological material at molecular resolution. Here, we summarize the most important sample preparation methods and technical aspects relevant for cryo-electron tomography, as well as its recent biological applications from isolated macromolecular complexes to entire cells and tissues.
© 2010 The Authors Journal of Microscopy © 2010 The Royal Microscopical Society.

Mesh：

Year:  2010        PMID： 21175615     DOI： 10.1111/j.1365-2818.2010.03478.x

Source DB:  PubMed          Journal:  J Microsc        ISSN： 0022-2720            Impact factor:   1.758


  17 in total

1.  Electron tomography of fiber cell cytoplasm and dense cores of multilamellar bodies from human age-related nuclear cataracts.

Authors:  M Joseph Costello; Alain Burette; Mariko Weber; Sangeetha Metlapally; Kurt O Gilliland; W Craig Fowler; Ashik Mohamed; Sönke Johnsen
Journal:  Exp Eye Res       Date:  2012-06-20       Impact factor: 3.467

2.  MBIR: A cryo-ET 3D reconstruction method that effectively minimizes missing wedge artifacts and restores missing information.

Authors:  Rui Yan; Singanallur V Venkatakrishnan; Jun Liu; Charles A Bouman; Wen Jiang
Journal:  J Struct Biol       Date:  2019-03-11       Impact factor: 2.867

Review 3.  Ultrastructural analysis of neuronal synapses using state-of-the-art nano-imaging techniques.

Authors:  Changlu Tao; Chenglong Xia; Xiaobing Chen; Z Hong Zhou; Guoqiang Bi
Journal:  Neurosci Bull       Date:  2012-08       Impact factor: 5.203

Review 4.  Correlative cryogenic tomography of cells using light and soft x-rays.

Authors:  Elizabeth A Smith; Bertrand P Cinquin; Myan Do; Gerry McDermott; Mark A Le Gros; Carolyn A Larabell
Journal:  Ultramicroscopy       Date:  2013-11-07       Impact factor: 2.689

Review 5.  Visualizing and quantifying cell phenotype using soft X-ray tomography.

Authors:  Gerry McDermott; Douglas M Fox; Lindsay Epperly; Modi Wetzler; Annelise E Barron; Mark A Le Gros; Carolyn A Larabell
Journal:  Bioessays       Date:  2012-01-30       Impact factor: 4.345

6.  Cryo-electron tomography for structural characterization of macromolecular complexes.

Authors:  Julia Cope; John Heumann; Andreas Hoenger
Journal:  Curr Protoc Protein Sci       Date:  2011-08

7.  Correlative microscopy methods that maximize specimen fidelity and data completeness, and improve molecular localization capabilities.

Authors:  Elizabeth A Smith; Bertrand P Cinquin; Gerry McDermott; Mark A Le Gros; Dilworth Y Parkinson; Hong Tae Kim; Carolyn A Larabell
Journal:  J Struct Biol       Date:  2013-03-24       Impact factor: 2.867

8.  Correlative VIS-fluorescence and soft X-ray cryo-microscopy/tomography of adherent cells.

Authors:  Christoph Hagen; Peter Guttmann; Barbara Klupp; Stephan Werner; Stefan Rehbein; Thomas C Mettenleiter; Gerd Schneider; Kay Grünewald
Journal:  J Struct Biol       Date:  2011-12-24       Impact factor: 2.867

Review 9.  Understanding the invisible hands of sample preparation for cryo-EM.

Authors:  Giulia Weissenberger; Rene J M Henderikx; Peter J Peters
Journal:  Nat Methods       Date:  2021-05-07       Impact factor: 47.990

10.  Cryo-electron tomography reveals a critical role of RIM1α in synaptic vesicle tethering.

Authors:  Rubén Fernández-Busnadiego; Shoh Asano; Ana-Maria Oprisoreanu; Eri Sakata; Michael Doengi; Zdravko Kochovski; Magdalena Zürner; Valentin Stein; Susanne Schoch; Wolfgang Baumeister; Vladan Lucić
Journal:  J Cell Biol       Date:  2013-05-27       Impact factor: 10.539
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