| Literature DB >> 21167704 |
Libing Wang1, Wenwei Ma, Wei Chen, Liqiang Liu, Wei Ma, Yingyue Zhu, Liguang Xu, Hua Kuang, Chuanlai Xu.
Abstract
An aptamer-based chromatographic strip assay method for rapid toxin detection was developed. The aptamer-based strip assay was based on the competition for the aptamer between ochratoxin A and DNA probes. The sensing results indicated that the sensitivity of the aptamer-based strip was better than that of conventional antibody-based strips. The visual limit of detection of the strip for qualitative detection was 1 ng/mL while the LOD for semi-quantitative detection could down to 0.18 ng/mL by using scanning reader. The recoveries of test samples were from 96% to 110%. All detections could be achieved in less than 10 min, indicating that the aptamer-based strip could be a potential useful tool for rapid on-site detections.Entities:
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Year: 2010 PMID： 21167704 DOI： 10.1016/j.bios.2010.11.040
Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618