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Literature DB >> 211488

Purification of a DNA nicking-closing enzyme from mouse L cells.

Abstract

A DNA nicking-closing enzyme has been purified from the nuclei of mouse L cells to 90% homogeneity. The denatured and reduced form of the enzyme has a molecular weight of 68,000 which is in agreement with the molecular weight of the native enzyme as determined by gel filtration and by sucrose sedimentation velocity assuming the protein is globular. Therefore, the active form of the enzyme is a monopolypeptide. Its isoelectric point is pH 4.2 +/- 0.2. The nicking-closing activity does not require a cofactor and does not involve any sulfhydryl group. The enzyme requires 0.2 M NaCl and pH in the range of 6.5-7.5 for optimal activity.

Entities: Chemical Species

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Year: 1978 PMID： 211488 PMCID： PMC342212 DOI： 10.1093/nar/5.8.2861

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

21 in total

1. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

Authors: F C GREENWOOD; W M HUNTER; J S GLOVER
Journal: Biochem J Date: 1963-10 Impact factor: 3.857

2. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors: R G MARTIN; B N AMES
Journal: J Biol Chem Date: 1961-05 Impact factor: 5.157

3. Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.

Authors: J J Champoux
Journal: Proc Natl Acad Sci U S A Date: 1977-09 Impact factor: 11.205

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. Purification and demonstration of the enzymatic character of the nicking-closing protein from mouse L cells.

Authors: H P Vosberg; J Vinograd
Journal: Biochem Biophys Res Commun Date: 1976-01-26 Impact factor: 3.575

6. An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).

Authors: J J Champoux; R Dulbecco
Journal: Proc Natl Acad Sci U S A Date: 1972-01 Impact factor: 11.205

7. Isoelectric analysis of neonatal monofocal antibody.

Authors: J L Press; N R Klinman
Journal: Immunochemistry Date: 1973-09

8. Mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerases. I. Purification and properties of an -amanitin-sensitive ribonucleic acid polymerase and stimulatory factors from HeLa and KB cells.

Authors: B Sugden; W Keller
Journal: J Biol Chem Date: 1973-06-10 Impact factor: 5.157

4. Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin.

Authors: A N Luchnik; V V Bakayev; I B Zbarsky; G P Georgiev
Journal: EMBO J Date: 1982 Impact factor: 11.598

4 in total

Purification of a DNA nicking-closing enzyme from mouse L cells.

1. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

2. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

3. Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.

4. Protein measurement with the Folin phenol reagent.

5. Purification and demonstration of the enzymatic character of the nicking-closing protein from mouse L cells.

6. An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).

7. Isoelectric analysis of neonatal monofocal antibody.

8. Mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerases. I. Purification and properties of an -amanitin-sensitive ribonucleic acid polymerase and stimulatory factors from HeLa and KB cells.

9. A DNA nicking-closing enzyme encapsidated in vaccinia virus: partial purification and properties.

10. Estimation of the molecular weights of proteins by Sephadex gel-filtration.

1. Eukaryotic DNA topoisomerases: two forms of type I DNA topoisomerases from HeLa cell nuclei.

2. DNA topoisomerase from Agrobacterium tumefaciens: purification and catalytic properties.

3. Drosophila topoisomerase I: isolation, purification and characterization.

4. Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin.