OBJECTIVES: This study aims to establish three-dimensional (3D) cell culture models of human ovarian and endometrial cancers and to compare biological and morphological characteristics of these models with those of two-dimensional (2D) models of the same cell lines and the primary tumours. METHODS: 3D models of ovarian and endometrial cancer cell cultures were established using a Rotary Cell Culture System. Immunohistochemical profiling and differential proteomics were used to characterize biological characteristics of multicellular spheroids (MCS) formed from these cultures. These were compared to characteristics of the same cells established in 2D and of the primary tumours from which the cell lines were derived. RESULTS: MCSs from 3D cell cultures appeared histologically similar to the primary tumours. Immunohistochemical profiling of multiple markers, including CA125, BCL2 and p53, showed that patterns of protein expression in MCSs resemble those of the primary tumours. Proteomic profiling identified several differentially expressed protein markers between 2D and 3D cultures. These included prohibitin, which was down-regulated in 3D cultures suggesting cells proliferate less compared to 2D cultures; and VDAC1 and annexin 4, which were up-regulated in 3D cultures suggesting greater levels of apoptosis in 3D compared to 2D models. CONCLUSION: Establishing 3D models of cancer cell lines is likely to be of value for studying the molecular and biological mechanisms of ovarian/endometrial tumour progression and for testing novel molecular targets for cancer therapy.
OBJECTIVES: This study aims to establish three-dimensional (3D) cell culture models of humanovarian and endometrial cancers and to compare biological and morphological characteristics of these models with those of two-dimensional (2D) models of the same cell lines and the primary tumours. METHODS: 3D models of ovarian and endometrial cancer cell cultures were established using a Rotary Cell Culture System. Immunohistochemical profiling and differential proteomics were used to characterize biological characteristics of multicellular spheroids (MCS) formed from these cultures. These were compared to characteristics of the same cells established in 2D and of the primary tumours from which the cell lines were derived. RESULTS: MCSs from 3D cell cultures appeared histologically similar to the primary tumours. Immunohistochemical profiling of multiple markers, including CA125, BCL2 and p53, showed that patterns of protein expression in MCSs resemble those of the primary tumours. Proteomic profiling identified several differentially expressed protein markers between 2D and 3D cultures. These included prohibitin, which was down-regulated in 3D cultures suggesting cells proliferate less compared to 2D cultures; and VDAC1 and annexin 4, which were up-regulated in 3D cultures suggesting greater levels of apoptosis in 3D compared to 2D models. CONCLUSION: Establishing 3D models of cancer cell lines is likely to be of value for studying the molecular and biological mechanisms of ovarian/endometrial tumour progression and for testing novel molecular targets for cancer therapy.
Authors: Jennifer J Winkenwerder; Patricia L Palechek; Julie S Reece; Mark A Saarinen; Mark A Arnold; Michael B Cohen; David W Murhammer Journal: Oncol Rep Date: 2003 Jul-Aug Impact factor: 3.906
Authors: H W Rhee; H E Zhau; S Pathak; A S Multani; S Pennanen; T Visakorpi; L W Chung Journal: In Vitro Cell Dev Biol Anim Date: 2001-03 Impact factor: 2.723
Authors: Dimitra Dafou; Barbara Grun; John Sinclair; Kate Lawrenson; Elizabeth C Benjamin; Estrid Hogdall; Susanne Kruger-Kjaer; Lise Christensen; Heidi M Sowter; Ahmed Al-Attar; Richard Edmondson; Stephen Darby; Andrew Berchuck; Peter W Laird; C Leigh Pearce; Susan J Ramus; Ian J Jacobs; Simon A Gayther Journal: Neoplasia Date: 2010-07 Impact factor: 5.715
Authors: Sarah E Kelly; Altomare Di Benedetto; Adelaide Greco; Candace M Howard; Vincent E Sollars; Donald A Primerano; Jagan V Valluri; Pier Paolo Claudio Journal: PLoS One Date: 2010-04-08 Impact factor: 3.240