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Literature DB >> 21131486

Canonical protein splicing of a class 1 intein that has a class 3 noncanonical sequence motif.

Abstract

A Thermobifida fusca intein has two characteristics of class 3 inteins: a noncontiguous covariant Trp-Cys-Thr triplet and a Ser flanking its C terminus. However, it has Cys at position one, characteristic of class 1 inteins. Splicing does not require the internal Cys, which may instead coordinate the active site. Therefore, the intein is class 1.

Entities: Chemical Species

Mesh：

Substances：
Bacterial Proteins

Year: 2010 PMID： 21131486 PMCID： PMC3028663 DOI： 10.1128/JB.01287-10

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

16 in total

1. A genetic system yields self-cleaving inteins for bioseparations.

Authors: D W Wood; W Wu; G Belfort; V Derbyshire; M Belfort
Journal: Nat Biotechnol Date: 1999-09 Impact factor: 54.908

2. InBase: the Intein Database.

Authors: Francine B Perler
Journal: Nucleic Acids Res Date: 2002-01-01 Impact factor: 16.971

Review 3. Protein splicing and related forms of protein autoprocessing.

Authors: H Paulus
Journal: Annu Rev Biochem Date: 2000 Impact factor: 23.643

4. CD-Search: protein domain annotations on the fly.

Authors: Aron Marchler-Bauer; Stephen H Bryant
Journal: Nucleic Acids Res Date: 2004-07-01 Impact factor: 16.971

5. Basic local alignment search tool.

Authors: S F Altschul; W Gish; W Miller; E W Myers; D J Lipman
Journal: J Mol Biol Date: 1990-10-05 Impact factor: 5.469

6. The Deinococcus radiodurans Snf2 intein caught in the act: detection of the Class 3 intein signature Block F branched intermediate.

Authors: Lear E Brace; Maurice W Southworth; Kazuo Tori; Michelle L Cushing; Francine Perler
Journal: Protein Sci Date: 2010-08 Impact factor: 6.725

Review 7. The mechanism of intein-mediated protein splicing: variations on a theme.

Authors: Kenneth V Mills; Francine B Perler
Journal: Protein Pept Lett Date: 2005-11 Impact factor: 1.890

8. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

Canonical protein splicing of a class 1 intein that has a class 3 noncanonical sequence motif.

1. A genetic system yields self-cleaving inteins for bioseparations.

2. InBase: the Intein Database.

Review 3. Protein splicing and related forms of protein autoprocessing.

4. CD-Search: protein domain annotations on the fly.

5. Basic local alignment search tool.

6. The Deinococcus radiodurans Snf2 intein caught in the act: detection of the Class 3 intein signature Block F branched intermediate.

Review 7. The mechanism of intein-mediated protein splicing: variations on a theme.

8. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

9. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

10. Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature.

Review 1. Recent progress in intein research: from mechanism to directed evolution and applications.

2. Expanding the definition of class 3 inteins and their proposed phage origin.

Review 3. Protein Splicing of Inteins: A Powerful Tool in Synthetic Biology.