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Literature DB >> 21115739

Rapid tagging and integration of genes in Giardia intestinalis.

Abstract

We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.

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Year: 2010 PMID： 21115739 PMCID： PMC3019802 DOI： 10.1128/EC.00190-10

Source DB: PubMed Journal: Eukaryot Cell ISSN： 1535-9786

16 in total

Review 1. Recycling the cell cycle: cyclins revisited.

Authors: Andrew W Murray
Journal: Cell Date: 2004-01-23 Impact factor: 41.582

2. Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination.

Authors: Bernd Schimanski; Tu N Nguyen; Arthur Günzl
Journal: Eukaryot Cell Date: 2005-11

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Journal: Gene Date: 1998-10-09 Impact factor: 3.688

4. Episomal and integrated maintenance of foreign DNA in Giardia lamblia.

Authors: S M Singer; J Yee; T E Nash
Journal: Mol Biochem Parasitol Date: 1998-04-01 Impact factor: 1.759

5. Stable coexpression of a drug-resistance gene and a heterologous gene in an ancient parasitic protozoan Giardia lamblia.

Authors: D C Yu; A L Wang; C C Wang
Journal: Mol Biochem Parasitol Date: 1996-12-02 Impact factor: 1.759

6. Development of a tetracycline controlled gene expression system in the parasitic protozoan Giardia lamblia.

Authors: C H Sun; J H Tai
Journal: Mol Biochem Parasitol Date: 2000-01-05 Impact factor: 1.759

7. The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome.

Authors: M Itaya; I Yamaguchi; K Kobayashi; T Endo; T Tanaka
Journal: J Biochem Date: 1990-06 Impact factor: 3.387

8. Stable DNA transfection of the primitive protozoan pathogen Giardia lamblia.

Authors: C H Sun; C F Chou; J H Tai
Journal: Mol Biochem Parasitol Date: 1998-04-01 Impact factor: 1.759

9. General purpose tagging vectors for fission yeast.

Authors: S L Forsburg; D A Sherman
Journal: Gene Date: 1997-06-03 Impact factor: 3.688

10. Stable expression of Escherichia coli beta-glucuronidase A (GusA) in Giardia lamblia: application to high-throughput drug susceptibility testing.

Authors: Joachim Müller; Dorothea Nillius; Adrian Hehl; Andrew Hemphill; Norbert Müller
Journal: J Antimicrob Chemother Date: 2009-10-10 Impact factor: 5.790

32 in total

1. Regulation of a Myb transcription factor by cyclin-dependent kinase 2 in Giardia lamblia.

Authors: Chao-Cheng Cho; Li-Hsin Su; Yu-Chang Huang; Yu-Jiao Pan; Chin-Hung Sun
Journal: J Biol Chem Date: 2011-12-13 Impact factor: 5.157

2. Nuclear inheritance and genetic exchange without meiosis in the binucleate parasite Giardia intestinalis.

Authors: Meredith L Carpenter; Zoe June Assaf; Stéphane Gourguechon; W Zacheus Cande
Journal: J Cell Sci Date: 2012-02-24 Impact factor: 5.285

3. Disc-associated proteins mediate the unusual hyperstability of the ventral disc in Giardia lamblia.

Authors: Christopher Nosala; Kari D Hagen; Nicholas Hilton; Tiffany M Chase; Kelci Jones; Rita Loudermilk; Kristofer Nguyen; Scott C Dawson
Journal: J Cell Sci Date: 2020-08-27 Impact factor: 5.285

4. Identification of obscure yet conserved actin-associated proteins in Giardia lamblia.

Authors: Alexander R Paredez; Arash Nayeri; Jennifer W Xu; Jana Krtková; W Zacheus Cande
Journal: Eukaryot Cell Date: 2014-04-11

5. The Giardia median body protein is a ventral disc protein that is critical for maintaining a domed disc conformation during attachment.

Authors: David J Woessner; Scott C Dawson
Journal: Eukaryot Cell Date: 2012-01-13

6. Hybrid Structured Illumination Expansion Microscopy Reveals Microbial Cytoskeleton Organization.

Authors: Aaron R Halpern; Germain C M Alas; Tyler J Chozinski; Alexander R Paredez; Joshua C Vaughan
Journal: ACS Nano Date: 2017-11-30 Impact factor: 15.881

7. The Giardia cell cycle progresses independently of the anaphase-promoting complex.

Authors: Stéphane Gourguechon; Liam J Holt; W Zacheus Cande
Journal: J Cell Sci Date: 2013-03-22 Impact factor: 5.285

8. Stable transfection of the diplomonad parasite Spironucleus salmonicida.

Authors: Jon Jerlström-Hultqvist; Elin Einarsson; Staffan G Svärd
Journal: Eukaryot Cell Date: 2012-09-14

9. Myosin-independent cytokinesis in Giardia utilizes flagella to coordinate force generation and direct membrane trafficking.

Authors: William R Hardin; Renyu Li; Jason Xu; Andrew M Shelton; Germain C M Alas; Vladimir N Minin; Alexander R Paredez
Journal: Proc Natl Acad Sci U S A Date: 2017-07-05 Impact factor: 11.205

10. Importance of enolase in Giardia lamblia differentiation.

Authors: Araceli Castillo-Romero; Barbara J Davids; Tineke Lauwaet; Frances D Gillin
Journal: Mol Biochem Parasitol Date: 2012-05-06 Impact factor: 1.759