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Literature DB >> 2118136

The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome.

M Itaya¹, I Yamaguchi, K Kobayashi, T Endo, T Tanaka.

Abstract

The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.

Entities: Chemical Species

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Year: 1990 PMID： 2118136 DOI： 10.1093/oxfordjournals.jbchem.a123128

Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387

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Cited

18 in total

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The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome.

1. Rapid tagging and integration of genes in Giardia intestinalis.

2. Physical distance between the site of type II DNA binding to the membrane and oriC on the Bacillus subtilis 168 chromosome.

3. Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene.

4. Role of enzymes of homologous recombination in illegitimate plasmid recombination in Bacillus subtilis.

5. Supramolecular nanofibers and hydrogels of nucleopeptides.

6. Mutants of Streptococcus suis types 1 and 2 impaired in expression of muramidase-released protein and extracellular protein induce disease in newborn germfree pigs.

7. Self-assembly of nucleopeptides to interact with DNAs.

8. Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.

9. Translation of trpG in Bacillus subtilis is regulated by the trp RNA-binding attenuation protein (TRAP).

10. Altered phosphorylation of Bacillus subtilis DegU caused by single amino acid changes in DegS.