Literature DB >> 21112402

Beyond the second virial coefficient: Sedimentation equilibrium in highly non-ideal solutions.

Germán Rivas1, Allen P Minton.   

Abstract

The general theory of sedimentation equilibrium (SE), applicable to mixtures of interacting sedimentable solutes at arbitrary concentration, is summarized. Practical techniques for the acquisition of SE data suitable for analysis are described. Experimental measurements and analyses of SE in concentrated protein solutions are reviewed. The method of non-ideal tracer sedimentation equilibrium (NITSE) is described. Experimental studies using NITSE to detect and quantitatively characterize intermolecular interactions in mixtures of dilute tracer species and concentrated proteins or polymers are reviewed.
Copyright © 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 21112402      PMCID： PMC3488769          DOI： 10.1016/j.ymeth.2010.11.004

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  37 in total

1.  Direct observation of the self-association of dilute proteins in the presence of inert macromolecules at high concentration via tracer sedimentation equilibrium: theory, experiment, and biological significance.

Authors:  G Rivas; J A Fernandez; A P Minton
Journal:  Biochemistry       Date:  1999-07-20       Impact factor: 3.162

2.  Hidden self-association of proteins.

Authors:  N Muramatsu; A P Minton
Journal:  J Mol Recognit       Date:  1989-04       Impact factor: 2.137

3.  Reversible self-association increases the viscosity of a concentrated monoclonal antibody in aqueous solution.

Authors:  Jun Liu; Mary D H Nguyen; James D Andya; Steven J Shire
Journal:  J Pharm Sci       Date:  2005-09       Impact factor: 3.534

4.  How can biochemical reactions within cells differ from those in test tubes?

Authors:  Allen P Minton
Journal:  J Cell Sci       Date:  2006-07-15       Impact factor: 5.285

5.  Quantitative characterization of weak self-association in concentrated solutions of immunoglobulin G via the measurement of sedimentation equilibrium and osmotic pressure.

Authors:  Mercedes Jiménez; Germán Rivas; Allen P Minton
Journal:  Biochemistry       Date:  2007-06-23       Impact factor: 3.162

6.  Patterns of protein protein interactions in salt solutions and implications for protein crystallization.

Authors:  André C Dumetz; Ann M Snellinger-O'brien; Eric W Kaler; Abraham M Lenhoff
Journal:  Protein Sci       Date:  2007-09       Impact factor: 6.725

Review 7.  Molecular crowding: analysis of effects of high concentrations of inert cosolutes on biochemical equilibria and rates in terms of volume exclusion.

Authors:  A P Minton
Journal:  Methods Enzymol       Date:  1998       Impact factor: 1.600

8.  Sedimentation equilibrium in macromolecular solutions of arbitrary concentration. II. Two protein components.

Authors:  R C Chatelier; A P Minton
Journal:  Biopolymers       Date:  1987-07       Impact factor: 2.505

9.  Rapid and accurate microfractionation of the contents of small centrifuge tubes: application in the measurement of molecular weight of proteins via sedimentation equilibrium.

Authors:  S Darawshe; G Rivas; A P Minton
Journal:  Anal Biochem       Date:  1993-02-15       Impact factor: 3.365

Review 10.  Protein-protein interactions: methods for detection and analysis.

Authors:  E M Phizicky; S Fields
Journal:  Microbiol Rev       Date:  1995-03
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  10 in total

1.  Variable-Field Analytical Ultracentrifugation: I. Time-Optimized Sedimentation Equilibrium.

Authors:  Jia Ma; Michael Metrick; Rodolfo Ghirlando; Huaying Zhao; Peter Schuck
Journal:  Biophys J       Date:  2015-08-18       Impact factor: 4.033

2.  Practical considerations over spectral quality in solid state NMR spectroscopy of soluble proteins.

Authors:  Marco Fragai; Claudio Luchinat; Giacomo Parigi; Enrico Ravera
Journal:  J Biomol NMR       Date:  2013-08-30       Impact factor: 2.835

3.  SedNMR: a web tool for optimizing sedimentation of macromolecular solutes for SSNMR.

Authors:  Lucio Ferella; Claudio Luchinat; Enrico Ravera; Antonio Rosato
Journal:  J Biomol NMR       Date:  2013-11-17       Impact factor: 2.835

4.  Association equilibrium of the HIV-1 capsid protein in a crowded medium reveals that hexamerization during capsid assembly requires a functional C-domain dimerization interface.

Authors:  Rebeca Bocanegra; Carlos Alfonso; Alicia Rodríguez-Huete; Miguel Ángel Fuertes; Mercedes Jiménez; Germán Rivas; Mauricio G Mateu
Journal:  Biophys J       Date:  2013-02-19       Impact factor: 4.033

5.  Crowding activates ClpB and enhances its association with DnaK for efficient protein aggregate reactivation.

Authors:  Ianire Martín; Garbiñe Celaya; Carlos Alfonso; Fernando Moro; Germán Rivas; Arturo Muga
Journal:  Biophys J       Date:  2014-05-06       Impact factor: 4.033

6.  Quantitative characterization of temperature-independent and temperature-dependent protein-protein interactions in highly nonideal solutions.

Authors:  Adedayo A Fodeke; Allen P Minton
Journal:  J Phys Chem B       Date:  2011-08-31       Impact factor: 2.991

Review 7.  Macromolecular Crowding In Vitro, In Vivo, and In Between.

Authors:  Germán Rivas; Allen P Minton
Journal:  Trends Biochem Sci       Date:  2016-09-23       Impact factor: 13.807

8.  Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation.

Authors:  Huaying Zhao; Chad A Brautigam; Rodolfo Ghirlando; Peter Schuck
Journal:  Curr Protoc Protein Sci       Date:  2013-02

9.  Analytical Ultracentrifugation as a Tool for Studying Protein Interactions.

Authors:  Peter Schuck
Journal:  Biophys Rev       Date:  2013-06-01

Review 10.  Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge.

Authors:  Stephen E Harding; Richard B Gillis; Fahad Almutairi; Tayyibe Erten; M Şamil Kök; Gary G Adams
Journal:  Biology (Basel)       Date:  2015-03-06
  10 in total

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