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Literature DB >> 2109186

Initiation and processing of two kappa immunoglobulin germ line transcripts in mouse B cells.

Abstract

The splicing patterns and sequences of two processed kappa immunoglobulin germ line mRNAs are presented. A 1.1-kilobase (kb) mRNA appeared to be derived from splicing of the previously characterized 8.4-kb germ line transcript, while a 0.8-kb mRNA was the splice product of a second 4.7-kb germ line transcript that initiated 50 base pairs upstream of J kappa 1. The interaction of the two kappa germ line promoters with nuclear binding factors is also examined. The potential role of these germ line transcripts in establishing the rearrangement potential of the locus is discussed.

Entities: Gene Species

Mesh：

Substances：

Year: 1990 PMID： 2109186 PMCID： PMC360541 DOI： 10.1128/mcb.10.5.1950-1958.1990

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

52 in total

1. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.

Authors: J C Alwine; D J Kemp; B A Parker; J Reiser; J Renart; G R Stark; G M Wahl
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

2. Identification and purification of a human lymphoid-specific octamer-binding protein (OTF-2) that activates transcription of an immunoglobulin promoter in vitro.

Authors: C Scheidereit; A Heguy; R G Roeder
Journal: Cell Date: 1987-12-04 Impact factor: 41.582

3. The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes.

Authors: E E Max; J V Maizel; P Leder
Journal: J Biol Chem Date: 1981-05-25 Impact factor: 5.157

Review 4. Somatic generation of antibody diversity.

Authors: S Tonegawa
Journal: Nature Date: 1983-04-14 Impact factor: 49.962

5. DNA between variable and joining gene segments of immunoglobulin kappa light chain is frequently retained in cells that rearrange the kappa locus.

Authors: B G Van Ness; C Coleclough; R P Perry; M Weigert
Journal: Proc Natl Acad Sci U S A Date: 1982-01 Impact factor: 11.205

10. The Downstream Transcriptional Enhancer, Ed, positively regulates mouse Ig kappa gene expression and somatic hypermutation.

Authors: Yougui Xiang; William T Garrard
Journal: J Immunol Date: 2008-05-15 Impact factor: 5.422

Initiation and processing of two kappa immunoglobulin germ line transcripts in mouse B cells.

1. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.

2. Identification and purification of a human lymphoid-specific octamer-binding protein (OTF-2) that activates transcription of an immunoglobulin promoter in vitro.

3. The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes.

Review 4. Somatic generation of antibody diversity.

5. DNA between variable and joining gene segments of immunoglobulin kappa light chain is frequently retained in cells that rearrange the kappa locus.

6. Early restriction of the human antibody repertoire.

7. A physical map of the DNA regions flanking the rabbit beta-globin gene.

8. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

9. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

10. Transcription of mouse kappa chain genes: implications for allelic exclusion.

1. V(D)J recombination is not activated by demethylation of the kappa locus.

2. A multistep mechanism for the activation of rearrangement in the immune system.

3. Regulation and a possible stage-specific function of Oct-2 during pre-B-cell differentiation.

4. Biallelic, ubiquitous transcription from the distal germline Ig{kappa} locus promoter during B cell development.

Review 5. Epigenetics of haematopoietic cell development.

6. Octamer independent activation of transcription from the kappa immunoglobulin germline promoter.

Review 7. Long-Range Regulation of V(D)J Recombination.

8. Role of the Igh intronic enhancer Eμ in clonal selection at the pre-B to immature B cell transition.

9. Stimulation of kappa light-chain gene rearrangement by the immunoglobulin mu heavy chain in a pre-B-cell line.

10. The Downstream Transcriptional Enhancer, Ed, positively regulates mouse Ig kappa gene expression and somatic hypermutation.