Literature DB >> 2108945

Expression of epidermal growth factor, transforming growth factor-alpha and their receptor genes in human gastric carcinomas; implication for autocrine growth.

K Yoshida1, E Kyo, T Tsujino, T Sano, M Niimoto, E Tahara.   

Abstract

The expressions of mRNA for epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha) and EGF receptor (EGFR) genes were examined in 7 human gastric carcinoma cell lines and 15 gastric carcinoma tissues and the corresponding normal mucosas. All of the gastric carcinoma cell lines expressed mRNA for EGFR and TGF-alpha genes. TMK-1 and MKN-28 cells also expressed EGF mRNA. Production of EGF, TGF-alpha and EGFR protein by gastric carcinoma cell lines was also confirmed by EGF and TGF-alpha specific monoclonal antibody binding. As for surgical specimens, EGFR and TGF-alpha mRNA were detected at high levels in all the tumor tissues. Interestingly, EGF mRNA was detected in 5 (33.3%) of the 15 gastric carcinomas but it was not detected in normal tissues. Moreover, anti-EGF and anti-TGF-alpha monoclonal antibodies inhibited the spontaneous 3H-TdR uptake by gastric carcinoma cells. These results suggest that EGF and/or TGF-alpha produced by tumor cells act as autocrine growth factors for gastric carcinomas.

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Year:  1990        PMID： 2108945      PMCID： PMC5917953          DOI： 10.1111/j.1349-7006.1990.tb02505.x

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN： 0910-5050


transforming growth factor‐α EGF, epidermal growth factor epidermal growth factor receptor monoclonal antibody standard saline citrate sodium dodecyl sulfate polyadenylated complementary DNA kilobase tumor/normal ratio
  46 in total

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Authors:  J J Smith; R Derynck; M Korc
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

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Journal:  Nature       Date:  1985 Jan 10-18       Impact factor: 49.962

3.  Growth-inhibitory effects of epidermal growth factor and overexpression of its receptors on human squamous cell carcinomas in culture.

Authors:  N Kamata; K Chida; K Rikimaru; M Horikoshi; S Enomoto; T Kuroki
Journal:  Cancer Res       Date:  1986-04       Impact factor: 12.701

4.  Evidence for the involvement of the submandibular gland epidermal growth factor in mouse mammary tumorigenesis.

Authors:  H Kurachi; S Okamoto; T Oka
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

5.  Synthesis of messenger RNAs for transforming growth factors alpha and beta and the epidermal growth factor receptor by human tumors.

Authors:  R Derynck; D V Goeddel; A Ullrich; J U Gutterman; R D Williams; T S Bringman; W H Berger
Journal:  Cancer Res       Date:  1987-02-01       Impact factor: 12.701

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Authors:  T J Velu; L Beguinot; W C Vass; M C Willingham; G T Merlino; I Pastan; D R Lowy
Journal:  Science       Date:  1987-12-04       Impact factor: 47.728

7.  Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization.

Authors:  G I Bell; N M Fong; M M Stempien; M A Wormsted; D Caput; L L Ku; M S Urdea; L B Rall; R Sanchez-Pescador
Journal:  Nucleic Acids Res       Date:  1986-11-11       Impact factor: 16.971

8.  Selective amplification of the cytoplasmic domain of the epidermal growth factor receptor gene in glioblastoma multiforme.

Authors:  L T Malden; U Novak; A H Kaye; A W Burgess
Journal:  Cancer Res       Date:  1988-05-15       Impact factor: 12.701

9.  Effect of human epidermal growth factor on cell growth and its receptor in human gastric carcinoma cell lines.

Authors:  A Ochiai; A Takanashi; N Takekura; K Yoshida; S Miyamori; T Harada; E Tahara
Journal:  Jpn J Clin Oncol       Date:  1988-03       Impact factor: 3.019

10.  Epidermal growth factor induces the expression of its receptor gene in human gastric carcinoma cell line TMK-1.

Authors:  K Yoshida; A Takanashi; E Kyo; M Ito; H Ito; M Niimoto; T Hattori; E Tahara
Journal:  Jpn J Cancer Res       Date:  1989-08
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  38 in total

1.  An Insufficient Preoperative Diagnosis of Borrmann Type 4 Gastric Cancer in Spite of EMR.

Authors:  Jae Bong Ahn; Tae Kyung Ha; Hang Rak Lee; Sung Joon Kwon
Journal:  J Gastric Cancer       Date:  2011-03-31       Impact factor: 3.720

2.  Expression of transforming growth factor alpha in human tissues: immunohistochemical study and northern blot analysis.

Authors:  W Yasui; Z Q Ji; H Kuniyasu; A Ayhan; H Yokozaki; H Ito; E Tahara
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1992

3.  Epidermal growth factor receptors in the oesophagus.

Authors:  J Jankowski; S Murphy; G Coghill; A Grant; K G Wormsley; D S Sanders; M Kerr; D Hopwood
Journal:  Gut       Date:  1992-04       Impact factor: 23.059

4.  Expression of growth factors and their receptors in human esophageal carcinomas: regulation of expression by epidermal growth factor and transforming growth factor alpha.

Authors:  K Yoshida; H Kuniyasu; W Yasui; Y Kitadai; T Toge; E Tahara
Journal:  J Cancer Res Clin Oncol       Date:  1993       Impact factor: 4.553

5.  Hypertrophic gastropathy resembling Ménétrier's disease in transgenic mice overexpressing transforming growth factor alpha in the stomach.

Authors:  H Takagi; C Jhappan; R Sharp; G Merlino
Journal:  J Clin Invest       Date:  1992-09       Impact factor: 14.808

6.  Correlation between epidermal growth factor receptor concentration and the growth of human gastric cancer xenografts in nude mice.

Authors:  T Kiyama; M Onda; A Tokunaga; I Fujita; T Okuda; T Mizutani; T Yoshiyuki; Y Shimizu; K Nishi; N Matsukura
Journal:  Gastroenterol Jpn       Date:  1992-08

7.  Expression of mRNA for epidermal growth factor, transforming growth factor-alpha and their receptor in human prostate tissue and cell lines.

Authors:  K Z Ching; E Ramsey; N Pettigrew; R D'Cunha; M Jason; J G Dodd
Journal:  Mol Cell Biochem       Date:  1993-09-22       Impact factor: 3.396

8.  Histamine H2-receptor antagonists stimulate proliferation but not migration of human gastric mucosal cells in vitro.

Authors:  C Ciacci; R Zarrilli; V Ricci; A De Luca; G Mazzacca; C Del Vecchio Blanco; M Romano
Journal:  Dig Dis Sci       Date:  1996-05       Impact factor: 3.199

9.  Relation of neuroendocrine cells to transforming growth factor-alpha and epidermal growth factor receptor expression in gastric adenocarcinomas: prognostic implications.

Authors:  Cigdem Celikel; Funda Eren; Bahadir Gulluoglu; Nural Bekiroglu; Serdar Turhal
Journal:  Pathol Oncol Res       Date:  2007-10-07       Impact factor: 3.201

10.  Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increases in the levels of epidermal growth factor and transforming growth factor alpha.

Authors:  M Korc; B Chandrasekar; Y Yamanaka; H Friess; M Buchier; H G Beger
Journal:  J Clin Invest       Date:  1992-10       Impact factor: 14.808
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