| Literature DB >> 2107074 |
D J Smith1, M K Burnham, J H Bull, J E Hodgson, J M Ward, P Browne, J Brown, B Barton, A J Earl, G Turner.
Abstract
A cosmid clone containing closely linked beta-lactam antibiotic biosynthetic genes was isolated from a gene library of Flavobacterium sp. SC 12,154. The location within the cluster of the DNA thought to contain the gene for delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS), the first step in the beta-lactam antibiotic biosynthetic pathway, was identified by a novel method. This DNA facilitated the isolation, by cross-hybridization, of the corresponding DNA from Streptomyces clavuligerus ATCC 27064, Penicillium chrysogenum Oli13 and Aspergillus nidulans R153. Evidence was obtained which confirmed that the cross-hybridizing sequences contained the ACVS gene. In each case the ACVS gene was found to be closely linked to other beta-lactam biosynthetic genes and constituted part of a gene cluster.Entities:
Mesh：
Substances：
Year: 1990 PMID： 2107074 PMCID： PMC551730 DOI： 10.1002/j.1460-2075.1990.tb08168.x
Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598