| Literature DB >> 2101685 |
E E Hood1, D H Clapham, I Ekberg, T Johannson.
Abstract
The hypervirulent Agrobacterium tumefaciens strain A281 formed frequent tumors (31%) on Picea abies (Norway spruce), an economically important tree species in Swedish forests. Three-month-old seedlings were inoculated and tumors were established that grew hormone-independently in culture. Tumors contained agropine and mannopine/mannopinic acid as determined by acid pH paper electrophoresis. In addition, DNA hybridization studies showed that the DNA from these tumor lines contained sequences homologous to Ti plasmid T-DNA, whereas wild-type spruce seedling DNA did not. These results suggest that Agrobacterium vectors can be used for gene transfer into this important forest species.Entities:
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Year: 1990 PMID： 2101685 DOI： 10.1007/bf00018552
Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076